Single-shot phase contrast microscopy using polarisation-resolved differential phase contrast

Kalita, Ranjan; Flanagan, William; Lightley, Jonathan; Kumar, Sunil; Alexandrov, Yurly; Garcia, Edwin; Hintze, Mark; Barkoulas, Michalis; Dunsby, Chris and French, Paul M.W. (2021). Single-shot phase contrast microscopy using polarisation-resolved differential phase contrast. Journal of Biophotonics, 14(12), article no. e202100144.

DOI: https://doi.org/10.1002/JBIO.202100144

Abstract

We present a robust, low-cost single-shot implementation of differential phase microscopy utilising a polarisation-sensitive camera to simultaneously acquire four images from which phase contrast
images can be calculated. This polarisation-resolved differential phase contrast (pDPC) microscopy technique can be easily integrated with fluorescence microscopy.

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