An Assessment of Stationary Phase Selectivity in SFC

Edge, Tony; James, Matt; Pipe, Colin; Bylikin, Sergey; Field, Jen and Euerby, Melvin (2022). An Assessment of Stationary Phase Selectivity in SFC. LCGC North America, 40(s6) pp. 9–22.



Supercritical fluid chromatography (SFC) has seen a recent resurgence in interest following investment in the development of instrument technology by numerous instrument manufacturers. Increased focus on sustainability in chromatographic science, coupled with the orthogonality to reversed phase HPLC, is likely to further drive the uptake of SFC in many sectors. As with any form of chromatography, optimizing separation selectivity is a key variable in providing adequate resolution and accurate identification and quantification of target analytes. Stationary phase chemistry can be readily exploited to substantially alter the separation selectivity obtained. This article examines and characterizes the selectivity differences offered by three prototype SFC phases.

Plain Language Summary

Three prototype HPLC phases (bare silica, cyanopropyl and 2-ethylpyridine) were evaluated for performance and selectivity under supercritical fluid chromatography (SFC) conditions. Clear selectivity differences were observed and quantified for 48 analytes with differing physicochemical properties. The selectivity values were substantially higher than those typically observed for reversed-phase columns. Stationary phase chemistry, therefore, was proved to be a powerful tool for optimizing the selectivity of SFC separations.

Viewing alternatives


Public Attention

Altmetrics from Altmetric

Number of Citations

Citations from Dimensions
No digital document available to download for this item

Item Actions