Optimization of Phytase Production from Escherichia coli by Altering Solid-State Fermentation Conditions

McKinney, Kyle; Combs, Justin; Becker, Patrick; Humphries, Andrea; Filer, Keith and Vriesekoop, Frank (2015). Optimization of Phytase Production from Escherichia coli by Altering Solid-State Fermentation Conditions. Fermentation, 1(1) pp. 13–23.

DOI: https://doi.org/10.3390/fermentation1010013

Abstract

Cultivation of Escherichia coli on wheat-bran substrate under various Solid-State Fermentation (SSF) conditions was evaluated for phytase yield along with the enzyme activity profile as a potential, low-cost alternative to submerged-liquid fermentation. The maximum phytase activity achieved by E. coli was 350 ± 50 SPU of phytase activity per gram of bran, incubated for 96 h with a substrate bed moisture content of 70% (w/v) at 37 °C with a relative air humidity of 90%, and supplemented with 10% (w/w bran) Luria-Bertani broth powder which translates into a 300% increase in phytase activity compared with an un-supplemented culture. The greatest improvements in phytase yield were associated with nutrient supplementation and the optimization of initial substrate moisture content. E. coli production of phytase utilizing solid-state fermentation technology was shown to be feasible utilizing the low-cost agro-residue wheat bran as substrate. Furthermore, the effect of pH and temperature on phytase activity was monitored from pH 2.5 to pH 7.5, and for temperatures ranging from 20 °C to 70 °C. Optimal phytase activity was at pH 5.5 and 50 °C when produced under the SSF optimized conditions.

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