Simulating the phase II metabolism of raloxifene on a screen-printed electrode

Vasiliadou, Rafaela and Welham, Kevin (2017). Simulating the phase II metabolism of raloxifene on a screen-printed electrode. Canadian Journal of Chemistry/Revue Canadienne de Chimie, 95(10) pp. 1085–1089.

DOI: https://doi.org/10.1139/cjc-2017-0279

Abstract

Raloxifene (RLX) is a selective estrogen receptor modulator widely used for the treatment of osteoporosis in post-menopause women. Toxicological in vitro studies suggested the reactivity of RLX through phase I metabolism. Herein, we describe a simple and inexpensive method for monitoring the reactive metabolism and detoxification of RLX by electrochemistry (EC) and mass spectrometry (MS). The phase I metabolite was synthesized electrochemically on a screen-printed electrode (SPE) and subsequently reacted with glutathione (GSH). The resulted GSH-adducts and GSH disulfides were characterized off-line by electrospray ionization (ESI)-MS.

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About

• Item ORO ID
• 71159
• Item Type
• Journal Item
• ISSN
• 1480-3291
• Keywords
• raloxifene; screen-printed electrodes; metabolism; toxicity
• Academic Unit or School
• Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences
  Faculty of Science, Technology, Engineering and Mathematics (STEM)
• Research Group
• Chemistry and Materials Research Group
• Copyright Holders
• © 2017 The Authors
• Related URLs
• • https://discovery.ucl.ac.uk/id/eprint/10...(Other)
• Depositing User
• Rafaela Vasiliadou