A Cell-Specific Analysis Of Gene Expression In The Neural Crest Of Zebrafish.

Van Roessel, Peter J. (1999). A Cell-Specific Analysis Of Gene Expression In The Neural Crest Of Zebrafish. MPhil thesis The Open University.

DOI: https://doi.org/10.21954/ou.ro.00010252

Abstract

This thesis presents a new approach to the rapid and systematic identification of genes expressed differentially between specific cell types. The approach is based upon the hybridization of amplified tissue or cell-specific complex cDNA probes to robotically arrayed cDNA libraries and the computer-assisted analysis ('electronic subtraction') of the resulting hybridization patterns.

This approach was applied to two populations of cultured zebrafish neural crest cells, as well as to tissue from ventral trunk neural tube. Sets of cDNA clones were assembled that putatively represent genes expressed in early-migrating trunk neural crest cells (390 clones), late-migrating trunk neural crest cells (422 clones), or both neural crest cell populations (292 clones), but not in ventral trunk neural tube.

The procedures and techniques used were tested for reproducibilty and initial conclusions are made about the feasibility and validity of the approach. A critical assessment of the techniques apphed suggests aspects of the approach which might be improved in its future application.

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