A novel role of Rab5 GTPase in chromosome alignment during mitosis in Drosophila melanogaster

Capalbo, Luisa (2011). A novel role of Rab5 GTPase in chromosome alignment during mitosis in Drosophila melanogaster. PhD thesis The Open University.

DOI: https://doi.org/10.21954/ou.ro.0000f8dc

Abstract

Rab5 is a conserved master regulator of membrane trafficking; it regulates the formation of early endosomes as well as their transport along microtubules and fusion to the target organelles. I identified Rab5 GTPase in a phenotypic screen for small GTPases involved during mitosis. In this thesis, I describe a novel role for Rab5 in cell division in Drosophila melanogaster. Inactivation of Rab5 by RNAi or mutation caused chromosome misalignment. Rab5 associated with Lamin in vivo and was required for the disassembly of the nuclear envelope at mitotic entry. Moreover, the release of the nuclear protein Mud, the Drosophila counterpart of NuMA, and its accumulation at the spindle poles was impaired in Rab5 RNAi cells. Consistent with this, Mud depletion phenocopied the Rab5 chromosome mis-alignment defect, confirming that this protein regulates spindle formation and chromosome congression like NuMA. My results unravel a novel role for Rab5 during mitosis and indicate that this GTPase is necessary for nuclear envelope disassembly at mitotic entry and proper distribution of spindle matrix components.

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