The Comparison of Different Methods for the Extraction of the Iminosugar Deoxynorjirimycin from Morus Alba

Sujangin, Norsuhadah; Sage, Edison; Abdullah, Zur Ain; Taib, Azney Zuhaily Md.; Kiappes, J.L.; Allman, Sarah; Zitzmann, Nicole; Nash, Robert J.; Mat, Nashriyah; Ali, Abdul Manaf and Mackeen, Mukram Mohamed (2017). The Comparison of Different Methods for the Extraction of the Iminosugar Deoxynorjirimycin from Morus Alba. In: The International Conference on Natural Products 2017, 15-16 Mar 2017, Swiss-Garden Beach Resort, Damai Laut, Perak, Malaysia.

Abstract

Deoxynojirimycin (DNJ) is polyhydroxylated alkaloid and iminosugar. It is a sugar analogue of D-glucose in which the oxygen atom in the ring is replaced by a nitrogen atom. The N-butyl (Misglustat) and hydroxyethyl (Miglitol) derivatives of DNJ are currently used clinically to treat Gaucher’s disease (a lysosomal storage disorder) and type II diabetes, respectively. The therapeutic iminosugar DNJ has been predominantly isolated from leaves of Morus alba (white mulberry) belonging to the Moraceae family. To date, there is a lack of studies comprehensively comparing the effects of different sample preparation methods on the extraction yields of DNJ. Therefore, M, alba leaves were used for a comparative study on the effects of different sample preparation conditions that included oven, room temperature, and liquid nitrogen-treatments and extraction using three different solvents (methanol, ethanol and acidified water) on the levels of DNJ in the leaves of M. alba. The samples were analysed quantitatively using ultra pressure liquid chromatography mass spectrometry (UPLC-MS) with selected ion recording of the pseudo molecular ion peak [M+H]+ 164 m/z at the retention time of 5.71 min. The UPLC-MS results suggested that ethanol extraction after oven drying was the method that yielded the highest content of DNJ.

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