A model for nanoparticle gene delivery systems

Bowen, James; Manickam, Mayandithevar; Rotov, Mikhail; Kendall, Kevin and Preece, Jon A. (2003). A model for nanoparticle gene delivery systems. In: ACORN Annual Event, 11-12 Nov 2003, Manchester, UK.

URL: https://www.academia.edu/9824470/A_model_for_nanop...


The objective of this research was to investigate nanoscale adhesion in systems which mimic non-viral gene delivery vectors. Specifically, chemically modified substrates which presented polyanionic surfaces (mimicking DNA) and polycationic surfaces (mimicking a nanoparticle with which to adhere the DNA) were manufactured. To do this, self-assembled monolayers (SAMs) of alkanethiols and dialkyl disulfides have been formed on thermally evaporated gold films, presenting a variety of chemically modified surfaces.

The wetting behaviour, elemental composition, thickness and surface topography of the SAMs were assessed. Only one of the SAM compounds was found to have not formed a SAM successfully. The compound contained a terminal quaternary pyridinium moiety, and it is believed the SAM formation was unsuccessful due to interaction between the iodide counterion and the gold surface.

The contact angle titration behaviour of the SAMs was investigated over the pH range 3-9, employing aqueous buffer solutions at high and low electrolyte concentrations. It was found that both pH and electrolyte concentration had little or no effect on the contact angle titration behaviour of the SAMs.

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