Systems for measuring B cell receptor affinity maturation in germinal centres

Müller, Thomas; Marshall, Jennifer; Dehghani, Hamid; Bowen, James and Toellner, Kai-Michael (2014). Systems for measuring B cell receptor affinity maturation in germinal centres. In: ID2 Inter-Disciplinary Inter-DTC, 26-27 Jun 2014, Oxford, UK.

URL: https://www.academia.edu/9854637/Systems_for_measu...

Abstract

In an effort to improve our understanding of the germinal centre (GC), this project aims to measure affinities of B cell receptors through the course of an immune response in order to map out spatial relationships of individual B cells during evolution in the GC. For this atomic force microscopy (AFM) and flow cytometry are being applied. Modifications to the AFM setup have been made to provide specificity towards the targeted receptors, and work on a range of known affinity B cells will demonstrate whether differences in affinities are distinguishable via AFM. Bespoke fluorescent probes have been prepared for use in flow cytometry to allow following affinity maturation during an immune response in large cell populations providing measures of relative affinity. When applied in histology this will also provide spatial information.

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