Structural analysis of isomeric N-glycans by ion mobility and negative ion fragmentation: applications to glycans released from fungi

Harvey, David J.; Allman, Sarah; Edgeworth, Matthew J. and Scrivens, James H. (2012). Structural analysis of isomeric N-glycans by ion mobility and negative ion fragmentation: applications to glycans released from fungi. In: American Society for Mass Spectrometry Annual Meeting 2012, 20-24 May 2012, Vancover, Canada.

Abstract

Little is known about the N-glycans present on the glycoproteins of basidiomycetes other than that they appear to consist mainly of the high-mannose glycans Man5-9GlcNAc2 with some other novel compounds present in a few species. The structures of the various possible isomers of the high-mannose glycans are largely unknown. This presentation describes the use of ion mobility and negative ion fragmentation for extracting the spectra of N-glycans from the complex mixtures resulting from N-glycan release from fungal glycoproteins and the use of both highly specific fragmentation of negative ions and of ion mobility for isomer identification.

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