Study on synthesis and fluorescence spectra of 5-bromor-4-thio-2′-deoxyuridine labeled with monobromobimanes fluorescein

Yin, Hong-yan; Zhang, Xiao-hui and Xu, Yao-zhong (2011). Study on synthesis and fluorescence spectra of 5-bromor-4-thio-2′-deoxyuridine labeled with monobromobimanes fluorescein. Journal of Molecular Science, 27(6) pp. 397–401.

URL: http://en.cnki.com.cn/Article_en/CJFDTotal-FZKB201...

Abstract

5-bromor-4-thio-2′-Deoxyuridine was synthesized by 1,2,4-triazole and thio-acetic acid from 5-bromor-2′-Deoxyuridine in mild condition,and then was carried out electrophilic reaction with monobromobimanes (mBBR)at 4 position sulphur,5-bromor-4-thio-2′-Deoxyuridine labeled with monobromobimanes (mBBR) was synthesized.Their structures were analyzed and characterized using 1 H NMR,MS,UV and fluorescence spectra.It can produce high fluorescence characteristics of the compound 5-bromo-4-thiodiamine-2′-deoxyuridine,which provides a body of 5-bromo-4-thio-2′-deoxyuridine-sentive detection method.