Characterization of antibody-antigen interactions by fluorescence spectroscopy

Missailidis, Sotiris and Brady, Kevin (2003). Characterization of antibody-antigen interactions by fluorescence spectroscopy. In: Lo, Benny and Chen, Yu Wai eds. Antibody engineering: methods and protocols. Methods in molecular biology (51). Totowa, NJ, USA: Humana Press, pp. 431–441.



This chapter presents the technical details for the study of antibody-antigen interactions using fluorescent spectroscopy. It identifies different methodologies for performing titrations depending on the availability and properties of the material, as well as providing information on ploting titration curves based on the quenching of the natural antibody fluorescence. Finally, it provides information on co-operativity and multiple antigen-antibody interactions and the way to handle those.

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