Targeting of Antibodies using Aptamers

Missailidis, Sotiris (2003). Targeting of Antibodies using Aptamers. In: Lo, Benny K.C. ed. Antibody engineering: methods and protocols. 2nd edition. Methods in Molecular Biology, 51. Humana Press, pp. 547–555.

DOI: https://doi.org/10.1385/1-59259-666-5:547

Abstract

The chapter presents a methodology for the rapid selection of aptamers against antibody targets. It is a detailed account of the various methodological steps that describe the selection of aptamers, including PCR steps, buffers to be used, target immobilisation, partitioning and amplification of aptamers, clonning and sequencing, to results in high affinity and specificity ligands for the chosen target antibody.

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About

• Item ORO ID
• 3878
• Item Type
• Book Section
• ISBN
• 1-58829-092-1, 978-1-58829-092-2
• Keywords
• aptamers; antibodies; aptamer selection; molecular biology techniques
• Academic Unit or School
• Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences
  Faculty of Science, Technology, Engineering and Mathematics (STEM)
• Depositing User
• Sotiris Missailidis