Targeting of Antibodies using Aptamers

Missailidis, Sotiris (2003). Targeting of Antibodies using Aptamers. In: Lo, Benny K.C. ed. Antibody engineering: methods and protocols. 2nd edition. Methods in Molecular Biology, 51. Humana Press, pp. 547–555.



The chapter presents a methodology for the rapid selection of aptamers against antibody targets. It is a detailed account of the various methodological steps that describe the selection of aptamers, including PCR steps, buffers to be used, target immobilisation, partitioning and amplification of aptamers, clonning and sequencing, to results in high affinity and specificity ligands for the chosen target antibody.

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