Loss of activity mutations in phospholipase C zeta (PLCζ) abolishes calcium oscillatory ability of human recombinant protein in mouse oocytes

Kashir, Junaid; Jones, Celine; Lee, Hoi Chang; Rietdorf, Katja; Nikiforaki, Dimitra; Durrans, Claire; Ruas, Margarida; Tee, Sze Tian; Heindryckx, Bjorn; Galione, Antony; De Sutter, Petra; Fissore, Rafael A.; Parrington, John and Coward, Kevin (2011). Loss of activity mutations in phospholipase C zeta (PLCζ) abolishes calcium oscillatory ability of human recombinant protein in mouse oocytes. Human Reproduction, 26(12) pp. 3372–87.

DOI: https://doi.org/10.1093/humrep/der336

URL: http://humrep.oxfordjournals.org/content/26/12/337...

Abstract

We demonstrate, for the first time, the production of active recombinant human PLCζ protein which retained the ability to elicit characteristic Ca(2+) oscillations in mouse oocytes, an ability which was eliminated by an infertility-linked mutation. These findings advance our understanding of PLCζ, and provide a critical step forward in obtaining purified PLCζ protein as a potential therapeutic agent for oocyte activation deficiency.

Viewing alternatives

Metrics

Item Actions

Export

You can export this page using these formats Digital Object Identifier - DOI