Ca₂₊-sensitive fluorescent dyes and intracellular Ca₂₊ imaging

Bootman, Martin D.; Rietdorf, Katja; Collins, Tony; Walker, Simon and Sanderson, Michael (2013). Ca₂₊-sensitive fluorescent dyes and intracellular Ca₂₊ imaging. Cold Spring Harbor Protocols, 2013(2), article no. pdb.top066050.

DOI: https://doi.org/10.1101/pdb.top066050

Abstract

Imaging Ca₂₊-sensitive fluorescent indicators provides a common approach for studying Ca₂₊ signals in many contexts. Fluorescent indicators are particularly useful for measuring acute Ca₂₊ changes in a relatively noninvasive manner. The availability of indicators that can be targeted to specific cellular domains, coupled with variations in affinity, brightness or spectral characteristics, provides tools for exploring spatially and temporally diverse Ca₂₊ signals, and moreover, multiplexing the readout of Ca₂₊ with other cellular functions. This article aims to give the novice experimentalist some insight into the considerations and potential pitfalls that impinge on the use of fluorescent Ca₂₊ indicators.

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