Electron transfer mechanism in Shewanella loihica PV-4 biofilms formed at graphite electrode

Jain, Anand; Zhang, Xiaoming; Pastorella, Gabriele; Connolly, Jack O.; Barry, Niamh; Woolley, Robert; Krishnamurthy, Satheesh and Marsili, Enrico (2012). Electron transfer mechanism in Shewanella loihica PV-4 biofilms formed at graphite electrode. Bioelectrochemistry, 87 pp. 28–32.

DOI: https://doi.org/10.1016/j.bioelechem.2011.12.012

Abstract

Electron transfer mechanisms in Shewanella loihica PV-4 viable biofilms formed at graphite electrodes were investigated in potentiostat-controlled electrochemical cells poised at oxidative potentials (0.2V vs. Ag/AgCl). Chronoamperometry (CA) showed a repeatable biofilm growth of S. loihica PV-4 on graphite electrode. CA, cyclic voltammetry (CV) and its first derivative shows that both direct electron transfer (DET) mediated electron transfer (MET) mechanism contributes to the overall anodic (oxidation) current. The maximum anodic current density recorded on graphite was 90μAcm^-2. Fluorescence emission spectra shows increased concentration of quinone derivatives and riboflavin in the cell-free supernatant as the biofilm grows. Differential pulse voltammetry (DPV) show accumulation of riboflavin at the graphite interface, with the increase in incubation period. This is the first study to observe a gradual shift from DET to MET mechanism in viable S. loihica PV-4 biofilms.

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