Normal Ca2+ signalling in glutathione-depleted and dithiothreitol-treated HeLa cells

Missiaen, Ludwig; Parys, Jan B.; Bootman, Martin D.; De Smedt, Humbert; Droogmans, Guy and Casteels, Rik (1993). Normal Ca2+ signalling in glutathione-depleted and dithiothreitol-treated HeLa cells. Pflügers Archiv, 423(5-6) pp. 480–484.



We have investigated whether reducing agents and substances that interfere with glutathione metabolism would affect the histamine-induced rises in internal Ca2+ concentration ([Ca2+]i) in indo-1-loaded HeLa cells. Individual cells responded to 1 microM histamine with either baseline or sinusoidal Ca2+ oscillations, a single Ca2+ peak or a maintained elevation of the [Ca2+]i. Only a few cells did not respond. The sulphydryl reducing agent dithiothreitol (5 mM) did not affect these responses to histamine. A 24-h preincubation with 1 mM DL-buthionine (SR)-sulphoximine, which reduces the cellular glutathione content to less than 20% of its control value, affected neither these histamine responses, nor the [Ca2+]i rises after application of 2 μM thapsigargin. We conclude that oxidation of critical sulphydryl groups is not required for the normal response to histamine and also that glutathione plays no role in agonist-induced Ca2+ signalling in HeLa cells.

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