Site-specific differences in fatty acid composition of dendritic cells and associated adipose tissue in Popliteal Depot, Mesentery, and Omentum and their modulation by chronic inflammation and dietary lipids

Mattacks, Christine A.; Sadler, Dawn and Pond, Caroline M. (2004). Site-specific differences in fatty acid composition of dendritic cells and associated adipose tissue in Popliteal Depot, Mesentery, and Omentum and their modulation by chronic inflammation and dietary lipids. Lymphatic Research and Biology, 2(3) pp. 107–129.

DOI: https://doi.org/10.1089/lrb.2004.2.107

URL: http://www.liebertonline.com/doi/abs/10.1089/lrb.2...

Abstract

Background: This study explores the role of lymphatics-associated adipocytes in determining the lipid composition of dendritic cells.

Methods and Results: Adult male rats were fed plain chow, or chow supplemented with 20% sunflower or fish oil. Chronic local inflammation was induced by subcutaneous injection of 20 µg lipopolysaccharide three times a week for 2 weeks near the popliteal lymph nodes. Chemokine-stimulated dendritic cells were collected over 4 h from popliteal and mesenteric lymph nodes, and perinodal and other samples of mesenteric, popliteal and omental adipose tissue. Fatty acids extracted from triacylglycerols and/or phospholipids were separated and quantified by gas chromatography from each sample of dendritic cells and intracellular lipids, membranes, stroma and isolated adipocytes from the adipose tissue. Dendritic cells from lymph nodes and adipose tissue samples differ in fatty acid composition, and can be modulated by diet. The site-specific differences of dendritic cells correlate with those of the contiguous adipocytes. Chronic mild stimulation alters the lipid composition of dendritic cells near the inflamed site and elsewhere; changes are minimal after the fish-oil diet. The composition of adipocyte triacylglycerol and phospholipid fatty acids also changes near the stimulation site in ways that counteract alterations induced by the experimental diets.

Conclusions: Fatty acids in dendritic cells differ with anatomical site, and are determined by the adjacent adipocytes, which actively regulate their own lipid composition. These findings demonstrate functional bases for the anatomical associations between adipose and lymphoid tissues and may be a mechanism by which dietary lipids modulate the immune system.

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