Desorption of small ionic fragments from oligonucleotides induced by low energy carbon ions

Ptasińska, S.; Mason, N. J.; Hunniford, C. A.; McCullough, R. W.; Denifl, A.; Mauracher, A. and Scheier, P. (2010). Desorption of small ionic fragments from oligonucleotides induced by low energy carbon ions. European Physical Journal D - Atomic, Molecular and Optical Physics, 60(1) pp. 59–63.

DOI: https://doi.org/10.1140/epjd/e2010-00083-x

Abstract

The degradation of oligonucleotide films containing differing base sequences induced by 4 keV C⁺ ions has been studied experimentally. The oligonucleotides were deposited onto a gold coated stainless steel substrate and the anions and cations released from the films were analyzed by a quadrupole mass spectrometer. The total ion desorption yield was recorded as a function of time using a constant C⁺ ion flux of 6 × 10¹⁴ ions cm^-2 s^-1. At low fluences the formation of small ionic fragments was observed, whilst for fluences greater than 1.2 × 10¹⁸ ions cm^-2 molecules were sputtered from the substrate. In addition to studies of the influence of a particular base to the total cation desorption yield, the effect of base substitution with bromine was measured for negative ion desorption. These results showed a strong degradation of oligonucleotide films during ion bombardment.

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