VUV irradiation studies of plasmid DNA in aqueous solution

Smialek, M. A.; Hoffman, S. V.; Folkard, M.; Prise, K. M.; Shuker, D. E. G.; Braithwaite, N. St. J. and Mason, N. J. (2008). VUV irradiation studies of plasmid DNA in aqueous solution. In: Radiation Damage in Biomolecular Systems (McGuigan, KG ed.), 19-22 Jul 2007, Dublin, p. 12020.

DOI: https://doi.org/10.1088/1742-6596/101/1/012020

URL: http://iopscience.iop.org/1742-6596/101/1/012020

Abstract

Interactions of VUV light and DNA samples in aqueous solutions are reported. The damage induced by such radiation is quantified by monitoring both loss of supercoiled DNA and formation of single and double strand breaks using agarose gel electrophoresis. Irradiations were performed using synchrotron VUV photons of 130, 150, 170 and 190 nm. VUV irradiation experiments revealed enhanced damage upon irradiation with 170 nm photons as compared with irradiations with photons of 150 nm and 130 nm. Irradiations carried at 190 nm caused the least damage.

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About

• Item ORO ID
• 24601
• Item Type
• Conference or Workshop Item
• ISSN
• 1742-6596
• Extra Information
• Annual Meeting of the COST P9 Action on Radiation Damage in Biomolecular Systems, Dublin, Ireland, JUL 19-22, 2007
  
  In Journal of Physics: Conference Series, vol.101, 2008
• Academic Unit or School
• Faculty of Science, Technology, Engineering and Mathematics (STEM) > Physical Sciences
  Faculty of Science, Technology, Engineering and Mathematics (STEM)
  Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences
• Research Group
• Physics
• Copyright Holders
• © 2008 IOP Publishing Ltd
• Depositing User
• N St.J Braithwaite