Unambiguous structural elucidation of base-modified purine nucleosides using NMR

Narukulla, Raman; Shuker, David E. G.; Ramesh, Vasudevan and Xu, Yao-Zhong (2008). Unambiguous structural elucidation of base-modified purine nucleosides using NMR. Magnetic Resonance in Chemistry, 46(1) pp. 1–8.

DOI: https://doi.org/10.1002/mrc.2111

Abstract

A general and unambiguous approach has been developed for structural elucidation of modified purine nucleosides using NMR spectroscopy. Systematic assignment of proton and carbon signals of modified nucleosides was firmly established by COSY and the anomerism of the glycosidic linkage of synthetic nucleosides clearly elucidated by NOESY experiments. Characteristic properties of 15N-isotopic labelling at specific positions of nucleosides were also employed for structural studies. The reported approach is applicable to othermodified nucleosides and nucleotides, as well as nucleobases.

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