Human Blood Brain Barrier Model

Couraud, P.O.; Romero, I.A. and Weksler, B.B. (2006). Human Blood Brain Barrier Model. INSERM; Cornell University; Couraud, P.O.; Romero, I.A.; Weksler, B.B., WO/2006/056879.

URL: http://www.wipo.int/pctdb/en/wo.jsp?wo=2006056879

Abstract

The present invention relates to an immortalized human brain endothelial cell line that is useful as an in vitro model of the blood brain barrier.
CLAIMS

1. An immortalized human brain endothelial cell line deposited at CNCM (Institut Pasteur) on October 7, 2004 under deposit number No. I-3308.

2. A cell from the cell line of claim 1.

3. A method of measuring blood brain barrier permeability of a test substance, which method comprises : - providing cells of claim 2 in one or more confluent monolayer(s) ;

- incubating said test substance with the confluent monolayer(s) ; and

- measuring the amount of said test substance permeated across the confluent monolayer(s).

4. The method of claim 3, wherein the amount of said test compound permeated across the confluent monolayer(s) is measured by passive diffusion or active transport.

5. A method for determining the toxicity of a test substance toward the blood brain barrier, which method comprises incubating said test substance with cells of claim 2 and assessing the viability of the cells.

6. Use of the cell line of claim 1 as an in vitro model of human blood brain barrier.

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