Amos, Claire; Romero, Ignacio A.; Schulze, Charlotte; Rousell, Jonathan; Pearson, Jeremy D.; Greenwood, John and Adamson, Peter
(2001).
| URL: | http://atvb.ahajournals.org/cgi/content/full/21/5/... |
|---|---|
| DOI (Digital Object Identifier) Link: | http://doi.org/10.1161/01.ATV.21.5.810 |
| Google Scholar: | Look up in Google Scholar |
Abstract
Intercellular adhesion molecule (ICAM)-1 plays a vital role in the process of leukocyte transmigration through endothelial cell (EC) barriers and has been shown to mediate signal transduction events in ECs induced either by its cross-linking or by the binding of T lymphocytes. Immunoblotting of ICAM-1 of Triton X-100 detergent fractions demonstrated that the majority of ICAM-1 was contained within the detergent-soluble fraction (noncytoskeletal associated) under basal conditions. After cross-linking of endothelial ICAM-1 with monoclonal antibody or coculture with T lymphocytes, EC ICAM-1 was observed to partition with a Triton X-100-insoluble (cytoskeletal associated) fraction in a dose- and time-dependent manner. Redistribution of ICAM-1 was specific, inasmuch as no association with the Triton X-100-insoluble fraction was observed after cross-linking of vascular cell adhesion molecule-1, nor did cross-linking of ICAM-1 result in a redistribution of the platelet and EC adhesion molecule. ICAM-1 association with the endothelial cytoskeleton after cross-linking was unaffected after treatment of the cells with cytochalasin D, C3-transferase, removal of extracellular calcium ions, or chelation of intracellular calcium ions. These data show that ICAM-1 colocalizes with the endothelial cytoskeleton and associates with a detergent-insoluble fraction after cross-linking.
| Item Type: | Journal Article |
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| Copyright Holders: | 2001 American Heart Association |
| Keywords: | endothelium; intercellular adhesion molecule-1; cytoskeleton |
| Academic Unit/Department: | Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences Faculty of Science, Technology, Engineering and Mathematics (STEM) |
| Interdisciplinary Research Centre: | Biomedical Research Network (BRN) |
| Item ID: | 8953 |
| Depositing User: | Ignacio A Romero |
| Date Deposited: | 22 Aug 2007 |
| Last Modified: | 02 Aug 2016 13:09 |
| URI: | http://oro.open.ac.uk/id/eprint/8953 |
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