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Ezrin and moesin co-localise with ICAM-1 in brain endothelial cells but are not directly associated

Romero, I.A.; Amos, C.L.; Greenwood, J. and Adamson, P. (2002). Ezrin and moesin co-localise with ICAM-1 in brain endothelial cells but are not directly associated. Molecular Brain Research, 105(1-2) pp. 47–59.

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The precise mechanism by which ICAM-1 transduces signals from adherent lymphocytes remains elusive. The ERM proteins ezrin and moesin were found to strongly co-localise with both ICAM-1 and F-actin in brain microvascular endothelial cells suggesting a potential role in mediating ICAM-1 signalling. Such strong co-localisation was maintained following treatment of cells with cytochalasin D, which inhibits actin polymerization and which is capable of inhibiting ICAM-1-induced signalling. Cross-linking of ICAM-1 demonstrated ICAM-1 clustering which no longer associated with ezrin or moesin. In addition immunoprecipitation analysis revealed that ICAM-1 was incapable of precipitating ERM proteins under conditions where ezrin was efficiently precipitated with anti-ICAM-2 antibodies. Fractionation of cell lysates on sucrose density gradients shows ICAM-1 and ezrin to sediment at different densities, whereas ICAM-2 co-sediments with ezrin. Together these data suggest that ICAM-1 is not directly associated with ezrin and moesin in brain microvascular endothelial cells.

Item Type: Journal Article
ISSN: 0169-328X
Keywords: CNS endothelium; ICAM-1; Cytoskeleton; ERM proteins; GP8 cells
Academic Unit/Department: Science > Life, Health and Chemical Sciences
Interdisciplinary Research Centre: Biomedical Research Network (BRN)
Item ID: 8952
Depositing User: Ignacio A Romero
Date Deposited: 23 Aug 2007
Last Modified: 07 Mar 2014 15:06
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