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Chemokine transport across human vascular endothelial cells

Mordelet, Elodie; Davies, Heather A.; Hillyer, Philippa; Romero, Ignacio A. and Male, David (2007). Chemokine transport across human vascular endothelial cells. Endothelium - Journal of Endothelial Cell Research, 14(1) pp. 7–15.

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Leukocyte migration across vascular endothelium is mediated by chemokines that are either synthesized by the endothelium or transferred across the endothelium from the tissue. The mechanism of transfer of two chemokines, CXCL10 (interferon gamma inducible protein [IP]-10) and CCL2 (macrophage chemotactic protein [MCP]-1), was compared across dermal and lung microvessel endothelium and saphenous vein endothelium. The rate of transfer depended on both the type of endothelium and the chemokine. The permeability coefficient (Pe) for CCL2 movement across saphenous vein was twice the value for dermal endothelium and four times that for lung endothelium. In contrast, the Pe value for CXCL10 was lower for saphenous vein endothelium than the other endothelia. The differences in transfer rate between endothelia was not related to variation in paracellular permeability using a paracellular tracer, inulin, and immunoelectron microscopy showed that CXCL10 was transferred from the basal membrane in a vesicular compartment, before distribution to the apical membrane. Although all three endothelia expressed high levels of the receptor for CXCL10 (CXCR3), the transfer was not readily saturable and did not appear to be receptor dependent. After 30 min, the chemokine started to be reinternalized from the apical membrane in clathrin-coated vesicles. The data suggest a model for chemokine transcytosis, with a separate pathway for clearance of the apical surface.

Item Type: Journal Item
ISSN: 1062-3329
Keywords: chemokines; chemokine receptors; endothelial cells; permeability; transcytosis
Academic Unit/School: Faculty of Science, Technology, Engineering and Mathematics (STEM)
Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences
Research Group: Innovation, Knowledge & Development research centre (IKD)
Item ID: 7623
Depositing User: Astrid Peterkin
Date Deposited: 08 May 2007
Last Modified: 12 Jun 2020 17:34
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