Development of Field Deployable Sensor for Detection of Pesticide from Food Chain

Nagabooshanam, Shalini; Sharma, Shruti; Roy, Souradeep; Mathur, Ashish; Krishnamurthy, Satheesh and Bharadwaj, Lalit M. (2020). Development of Field Deployable Sensor for Detection of Pesticide from Food Chain. IEEE Sensors Journal (Early Access).



The use of pesticides in agriculture field remains a serious issue related to public health. This necessitates the need to develop a low cost/portable, sensitive and selective bio-sensing platform for detection of pesticides in food chain. In this work a low-cost biosensing platform for ultrasensitive detection of chlorpyrifos (CPF) is developed. Electrochemical micro Paper Analytical Device (ElPAD) is fabricated by conventional screen-printing technology. Metal Organic Framework (Z1200 MOF) having zinc metal and imidazole ligand is used as a transducing element which facilitates biocompatible matrix for Acetylcholinesterase (AChE) enzyme immobilization. The limit of detection for CPF is found be 3 ng/L with sensitivity 0.521 kY/ng L-1/mm2. The sensor required 100lL of reagent and was tested with a linear concentration range of 10 ng/L to 1000 ng/L with response time of 5s. The sensor is further integrated with portable electronics based on Arduino microcontroller and Artificial Intelligence (AI) which provided economical, portable and user-friendly sensing platform. The stability of the sensor was 30 days. The developed sensor was also tested with real samples and the sensor response is in agreement with conventional technique.

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