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Monitoring type 2 diabetes from volatile faecal metabolome in Cushing's syndrome and single Afmid mouse models via a longitudinal study

Lourenco, Celia; Kelly, Darren; Cantillon, Jack; Cauchi, Michael; Yon, Marianne A; Bentley, Liz; Cox, Roger D and Turner, Claire (2019). Monitoring type 2 diabetes from volatile faecal metabolome in Cushing's syndrome and single Afmid mouse models via a longitudinal study. Scientific reports, 9(1), article no. 18779.

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DOI (Digital Object Identifier) Link: https://doi.org/10.1038/s41598-019-55339-9
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Abstract

The analysis of volatile organic compounds (VOCs) as a non-invasive method for disease monitoring, such as type 2 diabetes (T2D) has shown potential over the years although not yet set in clinical practice. Longitudinal studies to date are limited and the understanding of the underlying VOC emission over the age is poorly understood. This study investigated longitudinal changes in VOCs present in faecal headspace in two mouse models of T2D - Cushing's syndrome and single Afmid knockout mice. Longitudinal changes in bodyweight, blood glucose levels and plasma insulin concentration were also reported. Faecal headspace analysis was carried out using selected ion flow tube mass spectrometry (SIFT-MS) and thermal desorption coupled to gas chromatography-mass spectrometry (TD-GC-MS). Multivariate data analysis of the VOC profile showed differences mainly in acetic acid and butyric acid able to discriminate the groups Afmid and Cushing's mice. Moreover, multivariate data analysis revealed statistically significant differences in VOCs between Cushing's mice/wild-type (WT) littermates, mainly short-chain fatty acids (SCFAs), ketones, and alcohols, and longitudinal differences mainly attributed to methanol, ethanol and acetone. Afmid mice did not present statistically significant differences in their volatile faecal metabolome when compared to their respective WT littermates. The findings suggested that mice developed a diabetic phenotype and that the altered VOC profile may imply a related change in gut microbiota, particularly in Cushing's mice. Furthermore, this study provided major evidence of age-related changes on the volatile profile of diabetic mice.

Item Type: Journal Item
Copyright Holders: 2019 The Authors
ISSN: 2045-2322
Project Funding Details:
Funded Project NameProject IDFunding Body
PIMMS – Proton Ionization Molecular Mass Spectrometry287382European Union
Initial Training Networks (ITN)Not SetMarie Curie Actions
Not SetNot SetThe Open University (OU)
Academic Unit/School: Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences
Faculty of Science, Technology, Engineering and Mathematics (STEM)
Item ID: 68686
SWORD Depositor: Jisc Publications-Router
Depositing User: Jisc Publications-Router
Date Deposited: 10 Jan 2020 13:42
Last Modified: 17 Jan 2020 13:35
URI: http://oro.open.ac.uk/id/eprint/68686
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