Electrochemical micro analytical device interfaced with portable potentiostat for rapid detection of chlorpyrifos using acetylcholinesterase conjugated metal organic framework using Internet of things

Nagabooshanam, Shalini; Roy, Souradeep; Mathur, Ashish; Mukherjee, Irani; Krishnamurthy, Satheesh and Bharadwaj, Lalit (2019). Electrochemical micro analytical device interfaced with portable potentiostat for rapid detection of chlorpyrifos using acetylcholinesterase conjugated metal organic framework using Internet of things. Scientific Reports, 9, article no. 19862.

DOI: https://doi.org/10.1038/s41598-019-56510-y

Abstract

An Electrochemical micro Analytical Device (EµAD) was fabricated for sensitive detection of organophosphate pesticide chlorpyrifos in the food chain. Gold microelectrode (µE) modified with Zinc based Metal Organic Framework (MOF-Basolite Z1200) and Acetylcholinesterase (AChE) enzyme served as an excellent electro-analytical transducer for the detection of chlorpyrifos. Electrochemical techniques such as Cyclic Voltammetry (CV), Electrochemical Impedance Spectroscopy (EIS) and Differential Pulse Voltammetry (DPV) were performed for electrochemical analysis of the developed EµAD. The sensor needs only 2 µL of the analyte and it was tested within the linear range of 10 to 100 ng/L. The developed EµAD’s limit of detection (LoD) and sensitivity is 6 ng/L and 0.598 µ A/ng L⁻¹/mm² respectively. The applicability of the device for the detection of chlorpyrifos from the real vegetable sample was also tested within the range specified. The fabricated sensor showed good stability with a shelf-life of 20 days. The EµAD’s response time is of 50 s, including an incubation time of 20 s. The developed EµAD was also integrated with commercially available low-cost, handheld potentiostat (k-Stat) using Bluetooth and the results were comparable with a standard electrochemical workstation.

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About

• Item ORO ID
• 68642
• Item Type
• Journal Item
• ISSN
• 2045-2322
• Project Funding Details

• Funded Project Name	Project ID	Funding Body
  Plasma engineering of graphene and metal oxide functional layers for hybrid solar cells	IES\R2\170272	The Royal Society
  Integrated Treatment System for Textile Industry Wastewater	Not Set	Royal Academy of Engineering

• Keywords
• Food; Internet of things; carbon; metal oxides; lab on a chip
• Academic Unit or School
• Faculty of Science, Technology, Engineering and Mathematics (STEM) > Engineering and Innovation
  Faculty of Science, Technology, Engineering and Mathematics (STEM)
• Research Group
• Smart Materials
• Copyright Holders
• © 2019 The Authors
• Depositing User
• Satheesh Krishnamurthy