Daya, Sandeep; Loughlin, Jane and MacQueen, Hilary
Development of a three-dimensional co-culture system to study adipocyte and lymph node cell interactions.
In: British Society for Immunology Winter meeting 2005, 6-9 Dec 2005, Harrogate, UK.
White adipose tissue is involved in localised paracrine interactions with lymph node cells. In this adipocyte-lymph node relationship, localised immune challenges stimulate the release of Tumour Necrosis Factor-α, and Interleukins-4,6,8 and10. These cytokines direct neighbouring adipocytes to respond by releasing fatty acids from their triacylglycerol stores. This mechanism is believed to be of great importance as it provides energy and metabolic precursors to lymphoid cells during the immune response. The aim of the study is to investigate the activity exhibited by lymph node cells and adipocytes in a long term in vitro 3-dimensional co-culture system. A number of experimental approaches are being applied to successfully deliver lymph node cells and to increase their viability within the system.
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