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Complement component C4-like protein in Atlantic cod (Gadus morhua L.) - Detection in ontogeny and identification of post-translational deimination in serum and extracellular vesicles

Lange, Sigrun; Kraev, Igor; Magnadóttir, Bergljót and Dodds, Alister W. (2019). Complement component C4-like protein in Atlantic cod (Gadus morhua L.) - Detection in ontogeny and identification of post-translational deimination in serum and extracellular vesicles. Developmental & Comparative Immunology, 101, article no. 103437.

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DOI (Digital Object Identifier) Link: https://doi.org/10.1016/j.dci.2019.103437
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Abstract

The complement system is a critical part of teleost immune defences, with complement component C4 forming part of the classical and lectin pathways. Cod C4-like protein was isolated from plasma, specific antibodies generated and C4-like protein was assessed in cod sera, mucus and in extracellular vesicles (EVs) from serum and mucus. Higher levels of C4-like protein were detected in serum- than mucus-derived EVs. Post-translational deimination, caused by conversion of arginine into citrulline, can affect protein structure and function. Here we detected deiminated forms of C4-like protein in cod serum and at lower levels in mucus. C4-like protein was also found in deiminated form at low levels in EVs from both serum and mucus. C4-like protein was assessed by immunohistochemistry in cod larvae and detected in a range of organs including in liver, kidney, gut, muscle, skin and mucus, as well as in neuronal tissues of the brain, spinal cord and eye. This abundance of C4-like protein during early development may indicate roles in tissue remodelling, in addition to immune defences. The presence of deiminated C4-like protein in serum and mucosa, as well as in EVs, may suggest C4 protein moonlighting via post-translational deimination.

Item Type: Journal Item
ISSN: 0145-305X
Keywords: Complement C4; Protein deimination; Extracellular vesicles (EVs); Ontogeny;Cod (Gadus morhua L.)
Academic Unit/School: Faculty of Science, Technology, Engineering and Mathematics (STEM)
Item ID: 62388
Depositing User: ORO Import
Date Deposited: 10 Jul 2019 11:50
Last Modified: 19 Sep 2019 23:25
URI: http://oro.open.ac.uk/id/eprint/62388
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