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Deiminated proteins in extracellular vesicles and plasma of nurse shark (Ginglymostoma cirratum) - Novel insights into shark immunity

Criscitiello, Michael F.; Kraev, Igor and Lange, Sigrun (2019). Deiminated proteins in extracellular vesicles and plasma of nurse shark (Ginglymostoma cirratum) - Novel insights into shark immunity. Fish & Shellfish Immunology, 92 pp. 249–255.

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DOI (Digital Object Identifier) Link: https://doi.org/10.1016/j.fsi.2019.06.012
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Abstract

Peptidylarginine deiminases (PADs) are phylogenetically conserved calcium-dependent enzymes which post-translationally convert arginine into citrulline in target proteins in an irreversible manner, causing functional and structural changes in target proteins. Protein deimination causes generation of neo-epitopes, affects gene regulation and also allows for protein moonlighting. Extracellular vesicles are found in most body fluids and participate in cellular communication via transfer of cargo proteins and genetic material. In this study, post-translationally deiminated proteins and extracellular vesicles (EVs) are described for the first time in shark plasma. We report a poly-dispersed population of shark plasma EVs, positive for phylogenetically conserved EV-specific markers and characterised by TEM. In plasma, 6 deiminated proteins, including complement and immunoglobulin, were identified, whereof 3 proteins were found to be exported in plasma-derived EVs. A PAD homologue was identified in shark plasma by Western blotting and detected an expected 70 kDa size. Deiminated histone H3, a marker of neutrophil extracellular trap formation, was also detected in nurse shark plasma. This is the first report of deiminated proteins in plasma and EVs, highlighting a hitherto unrecognized post-translational modification in key immune proteins of innate and adaptive immunity in shark.

Item Type: Journal Item
Copyright Holders: 2019 Elsevier Ltd.
ISSN: 1050-4648
Keywords: Peptidylarginine deiminases (PADs); Protein deimination; Shark (Ginglymostoma cirratum); Extracellular vesicles (EVs); Innate immunity; Adaptive immunity; Complement C3; Novel antigen receptor (NAGR); IgW; Alpha 2-macroglobulin; Hemopexin; Haptoglobin
Academic Unit/School: Faculty of Science, Technology, Engineering and Mathematics (STEM)
Related URLs:
Item ID: 62181
Depositing User: ORO Import
Date Deposited: 26 Jun 2019 09:41
Last Modified: 19 Sep 2019 23:21
URI: http://oro.open.ac.uk/id/eprint/62181
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