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Impact of Repetitive Mild Traumatic Brain Injury on Behavioral and Hippocampal Deficits in a Mouse Model of Chronic Stress

Algamal, Moustafa; Saltiel, Nicole; Pearson, Andrew J.; Ager, Bryan; Burca, Ioana; Mouzon, Benoit; Diamond, David M.; Mullan, Michael; Ojo, Joseph O. and Crawford, Fiona (2019). Impact of Repetitive Mild Traumatic Brain Injury on Behavioral and Hippocampal Deficits in a Mouse Model of Chronic Stress. Journal of Neurotrauma, 36(17) pp. 2590–2607.

DOI (Digital Object Identifier) Link: https://doi.org/10.1089/neu.2018.6314
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Abstract

Clinical studies examining the interaction between traumatic brain injury (TBI) and stress-related disorders (e.g., post-traumatic stress disorder) are often complicated by methodological constraints, such as heterogeneity in injury type and severity, time post-trauma, and predisposing risk factors. Developing relevant animal models whereby many variables can be efficiently controlled is thus essential to understanding this elusive relationship. Here, we use our repeated unpredictable stress (RUS) paradigm, in combination with our established mouse model of repetitive mild TBI (r-mTBI), to assess the impact of repeated exposures to these paradigms on behavioral and neurobiological measures. C57BL/6J male mice were exposed to RUS and r-mTBI at 3 and 6 months of age followed by batteries of behavioral testing. Mice were euthanized 10 days and 3 months post-exposure, with brain and plasma samples collected for molecular profiling. The RUS paradigm involved exposure to a predator odor (trimethylthiazoline; TMT) while under restraint, daily unstable social housing, five inescapable footshocks on separate days, and chronic social isolation. Animals receiving r-mTBI ( × 5) and stress were exposed to a single closed-head injury 1 h after each footshock. Stress-alone mice showed significant weight loss, recall of traumatic memories, and anxiety-like and passive stress-coping behavior when compared with control mice. However, in stress+r-mTBI animals, the changes in cued fear memory, anxiety, and stress-coping tests were diminished, possibly due to TBI-induced hyperactivity. We also report complex brain molecular and neuropathological findings. Stress and r-mTBI, either individually or comorbidly, were associated with a chronic reduction in dendritic spine GluN2A/GluN2B ratio in the hippocampus. While stress augmented the r-mTBI–dependent astrogliosis in the corpus callosum, it mitigated r-mTBI–induced increases in hippocampal pro–brain-derived neurotrophic factor. We anticipate that our model will be a good platform to untangle the complex comorbid pathophysiology in stress disorders and r-mTBI.

Item Type: Journal Item
Copyright Holders: 2019 Mary Ann Liebert, Inc.
ISSN: 0897-7151
Keywords: animal studies, behavior, locomotor function, neuroplasticity, traumatic brain injury
Academic Unit/School: Faculty of Science, Technology, Engineering and Mathematics (STEM)
Item ID: 61975
Depositing User: ORO Import
Date Deposited: 19 Jun 2019 08:10
Last Modified: 18 Sep 2019 15:20
URI: http://oro.open.ac.uk/id/eprint/61975
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