The Open UniversitySkip to content

Extracellular Vesicles from Cod (Gadus morhua L.) Mucus contain Innate Immune Factors and Deiminated Protein Cargo

Magnadóttir, Bergljót; Kraev, Igor; Guðmundsdóttir, Sigríður; Dodds, Alister W. and Lange, Sigrun (2019). Extracellular Vesicles from Cod (Gadus morhua L.) Mucus contain Innate Immune Factors and Deiminated Protein Cargo. Developmental & Comparative Immunology, 99, article no. 103397.

Full text available as:
Full text not publicly available (Accepted Manuscript)
Due to publisher licensing restrictions, this file is not available for public download until 16 May 2020
Click here to request a copy from the OU Author.
DOI (Digital Object Identifier) Link:
Google Scholar: Look up in Google Scholar


Extracellular vesicles are released from cells and participate in cell communication via transfer of protein and genetic cargo derived from the parent cells. EVs play roles in normal physiology and immunity and are also linked to various pathological processes. Peptidylarginine deiminases (PADs) are phylogenetically conserved enzymes with physiological and pathophysiological roles. PADs cause post-translational protein deimination, resulting in structural and, in some cases, functional changes in target proteins and are also linked to EV biogenesis. This study describes for the first time EVs isolated from cod mucosa. Mucosal EVs were characterised by electron microscopy, nanoparticle tracking analysis and EV-specific surface markers. Cod mucosal EVs were found to carry PAD, complement component C3 and C-reactive proteins. C3 was found to be deiminated in both whole mucus and mucosal EVs, with some differences, and further 6 deiminated immune and cytoskeletal proteins were identified in EVs by LC-MS/MS analysis. As mucosal surfaces of teleost fish reflect human mucosal surfaces, these findings may provide useful insights into roles of EVs in mucosal immunity throughout phylogeny.

Item Type: Journal Item
Copyright Holders: 2019 Elsevier Ltd.
ISSN: 0145-305X
Keywords: extracellular vesicles (EVs); mucosal immunity; peptidylarginine deiminase (PAD); protein deimination; complement C3; CRP; cod (Gadus morhua L.)
Academic Unit/School: Faculty of Science, Technology, Engineering and Mathematics (STEM)
Item ID: 61409
Depositing User: ORO Import
Date Deposited: 22 May 2019 13:25
Last Modified: 10 Jun 2019 17:10
Share this page:


Altmetrics from Altmetric

Citations from Dimensions

Actions (login may be required)

Policies | Disclaimer

© The Open University   contact the OU