Precise generation of selective surface-confined glycoprotein recognition sites

Mitchell, Philippa; Tommasone, Stefano; Angioletti-Uberti, Stefano; Bowen, James and Mendes, Paula M (2019). Precise generation of selective surface-confined glycoprotein recognition sites. ACS Applied Bio Materials, 2(6) pp. 2617–2623.

DOI: https://doi.org/10.1021/acsabm.9b00289

Abstract

Since glycoproteins have become increasingly recognized as key players in a wide variety of disease processes, there is an increasing need for advanced affinity materials for highly selective glycoprotein binding. Herein, for the first time, a surface-initiated controlled radical polymerization is integrated with supramolecular templating and molecular imprinting to yield highly reproducible synthetic recognition sites on surfaces with dissociation constants (KDs) in the low micromolar range for target glycoproteins and minimal binding to non-target glycoproteins. Importantly, it is shown that the synthetic strategy has remarkable ability to distinguish the glycosylated and non-glycosylated forms of the same glycoprotein, with >5-fold difference in binding affinity. The precise control over the polymer film thickness and positioning of multiple carbohydrate receptors plays a crucial role in achieving enhanced affinity and selectivity. The generated functional materials of unprecedented glycoprotein recognition performance open up a wealth of opportunities in the biotechnological and biomedical fields.

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