The Open UniversitySkip to content
 

Precise generation of selective surface-confined glycoprotein recognition sites

Mitchell, Philippa; Tommasone, Stefano; Angioletti-Uberti, Stefano; Bowen, James and Mendes, Paula M (2019). Precise generation of selective surface-confined glycoprotein recognition sites. ACS Applied Bio Materials (Early Access).

Full text available as:
Full text not publicly available (Accepted Manuscript)
Due to publisher licensing restrictions, this file is not available for public download until 13 May 2020
Click here to request a copy from the OU Author.
DOI (Digital Object Identifier) Link: https://doi.org/10.1021/acsabm.9b00289
Google Scholar: Look up in Google Scholar

Abstract

Since glycoproteins have become increasingly recognized as key players in a wide variety of disease processes, there is an increasing need for advanced affinity materials for highly selective glycoprotein binding. Herein, for the first time, a surface-initiated controlled radical polymerization is integrated with supramolecular templating and molecular imprinting to yield highly reproducible synthetic recognition sites on surfaces with dissociation constants (KDs) in the low micromolar range for target glycoproteins and minimal binding to non-target glycoproteins. Importantly, it is shown that the synthetic strategy has remarkable ability to distinguish the glycosylated and non-glycosylated forms of the same glycoprotein, with >5-fold difference in binding affinity. The precise control over the polymer film thickness and positioning of multiple carbohydrate receptors plays a crucial role in achieving enhanced affinity and selectivity. The generated functional materials of unprecedented glycoprotein recognition performance open up a wealth of opportunities in the biotechnological and biomedical fields.

Item Type: Journal Item
Copyright Holders: 2019 American Chemical Society
Keywords: glycoprotein recognition; selectivity; molecular imprinting; radical polymerization
Academic Unit/School: Faculty of Science, Technology, Engineering and Mathematics (STEM) > Engineering and Innovation
Faculty of Science, Technology, Engineering and Mathematics (STEM)
Research Group: Smart Materials
Item ID: 61254
Depositing User: James Bowen
Date Deposited: 17 May 2019 09:53
Last Modified: 31 May 2019 21:47
URI: http://oro.open.ac.uk/id/eprint/61254
Share this page:

Metrics

Altmetrics from Altmetric

Citations from Dimensions

Actions (login may be required)

Policies | Disclaimer

© The Open University   contact the OU