The Open UniversitySkip to content

Gene expression profiling of bone cells on smooth and rough titanium surfaces

Harle, J.; Salih, V.; Olsen, I.; Brett, P.M.; Jones, F.H. and Tonetti, M. (2004). Gene expression profiling of bone cells on smooth and rough titanium surfaces. Journal of Materials Science: Materials in Medicine, 15(11) pp. 1255–1258.

Full text available as:
PDF (Accepted Manuscript) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
Download (1MB)
DOI (Digital Object Identifier) Link:
Google Scholar: Look up in Google Scholar


Titanium (Ti) and Ti alloys are widely used as dental and orthopaedic implants, but the effects of the surface characteristics of these materials on the response of cells and target tissues is not well understood. The present study has therefore examined the effects of a rough Ti (RT) and a smooth Ti (ST) surface on human bone cells in vitro. Scanning electron microscopy showed attachment and spreading of cells on both surfaces. Expression profiling using ATLAS™ gene arrays showed marked differences in gene responses after 3 h of culture. A number of osteoblast genes were identified as "roughness response" genes on the basis of changes in expression on the RT compared with the ST surfaces. The surface roughness of Ti was thus found to have a profound effect on the profile of genes expressed by the bone cells, and suggests that improvements in the biological activity and possibly the clinical efficacy of these materials could be achieved by selective regulation of gene expression mediated by controlled modification of Ti surface.

Item Type: Journal Item
Copyright Holders: 2004 Kluwer Academic Publishers
ISSN: 1573-4838
Extra Information: The original publication is available at
Keywords: Titanium; surface; bone cells; gene expression
Academic Unit/School: Faculty of Science, Technology, Engineering and Mathematics (STEM) > Physical Sciences
Faculty of Science, Technology, Engineering and Mathematics (STEM)
Item ID: 6015
Depositing User: Jamie Harle
Date Deposited: 12 Dec 2006
Last Modified: 08 Dec 2018 06:53
Share this page:


Altmetrics from Altmetric

Citations from Dimensions

Download history for this item

These details should be considered as only a guide to the number of downloads performed manually. Algorithmic methods have been applied in an attempt to remove automated downloads from the displayed statistics but no guarantee can be made as to the accuracy of the figures.

Actions (login may be required)

Policies | Disclaimer

© The Open University   contact the OU