The Open UniversitySkip to content

Initial responses of human osteoblasts to sol–gel modified titanium with hydroxyapatite and titania composition

Harle, Jamie; Kim, Hae-Won; Mordan, Nicky; Knowles, Jonathan C. and Salih, Vehid (2006). Initial responses of human osteoblasts to sol–gel modified titanium with hydroxyapatite and titania composition. Acta Biomaterialia, 2(5) pp. 547–556.

Full text available as:
PDF (Accepted Manuscript) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
Download (1MB) | Preview
DOI (Digital Object Identifier) Link:
Google Scholar: Look up in Google Scholar


Sol–gel thin films of hydroxyapatite (HA) and titania (TiO2) have received a great deal of attention in the area of bioactive surface modification of titanium (Ti) implants. Sol–gel coatings were developed on Ti substrates of pure HA and TiO2 and two composite forms, HA + 10% TiO2 and HA + 20% TiO2, and the biological properties of the coatings were evaluated. All the coating layers exhibited thin and homogeneous structures and phase-pure compositions (either HA or TiO2). Primary human osteoblast cells showed good attachment, spreading and proliferation on all the sol–gel coated surfaces, with enhanced cell numbers on all the coated surfaces relative to uncoated Ti control at day 1, as observed by MTT assay and scanning electron microscopy. Cell attachment rates were also enhanced on the pure HA coating relative to control Ti. The pure HA and HA + 10% TiO2 composite coating furthermore enhanced proliferation of osteoblasts at 4 days. Moreover, the gene expression level of several osteogenic markers including bone sialoprotein and osteopontin, as measured by RT–PCR at 24 h, was shown to vary according to coating composition. These findings suggest that human primary bone cells show marked and rapid early functional changes in response to HA and TiO2 sol–gel coatings on Ti.

Item Type: Journal Item
Copyright Holders: 2006 Acta Materialia Inc.
ISSN: 1742-7061
Keywords: titania; hydroxyapatite; sol–gel coating; titanium substrate; osteoblast
Academic Unit/School: Faculty of Science, Technology, Engineering and Mathematics (STEM) > Physical Sciences
Faculty of Science, Technology, Engineering and Mathematics (STEM)
Item ID: 6012
Depositing User: Jamie Harle
Date Deposited: 12 Dec 2006
Last Modified: 02 May 2018 12:40
Share this page:


Altmetrics from Altmetric

Citations from Dimensions

Download history for this item

These details should be considered as only a guide to the number of downloads performed manually. Algorithmic methods have been applied in an attempt to remove automated downloads from the displayed statistics but no guarantee can be made as to the accuracy of the figures.

Actions (login may be required)

Policies | Disclaimer

© The Open University   contact the OU