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Low Fluorescence Enzyme Matrices Based on Microfabricated SU-8 Films for a Phenol Micro-Biosensor Application

Psoma, Sotiria D.; van der Wal, Peter D. and de Rooij, Nico F. (2011). Low Fluorescence Enzyme Matrices Based on Microfabricated SU-8 Films for a Phenol Micro-Biosensor Application. Procedia Engineering, 25 pp. 1369–1372.

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DOI (Digital Object Identifier) Link: https://doi.org/10.1016/j.proeng.2011.12.338
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Abstract

In this contribution, the possibility of using SU-8 photoresist, a polymer widely used in MEMS applications, for the development of inexpensive and disposable optical phenol micro-biosensors is explored. The immobilisation of the enzyme, the encapsulation of the indicator and the patterning of the SU-8 were accomplished simultaneously in a simple one step microfabrication process. The enzyme still showed activity after encapsulation in SU-8 although the process involved its embedding in a hard and rigid epoxy resin matrix. This was carried out by measuring the signal of an oxygen-sensitive indicator (ruthenium-complex) through monitoring of the enzymatic oxidation of phenol which consumes oxygen. Films without enzyme showed negligible variation in fluorescence intensity upon phenol addition, whereas films with encapsulated enzyme and oxygen-sensitive fluorescent indicators showed a very clear change in fluorescence intensity upon addition of phenol. The current work demonstrates a new concept of a low cost immobilisation technique in combination with the microfabrication process for biosensor technology.

Item Type: Journal Item
Copyright Holders: 2011 Elsevier Ltd.
ISSN: 1877-7058
Keywords: SU-8 films; microfabrication; immobilisation; tyrosinase; phenol biosensor; fluorescence
Academic Unit/School: Faculty of Science, Technology, Engineering and Mathematics (STEM) > Engineering and Innovation
Faculty of Science, Technology, Engineering and Mathematics (STEM)
Research Group: Smart Materials
Item ID: 60024
Depositing User: Sotiria Psoma
Date Deposited: 26 Mar 2019 13:43
Last Modified: 01 Apr 2019 03:01
URI: http://oro.open.ac.uk/id/eprint/60024
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