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Sniffing Out Resistance - Rapid Identification of Urinary Tract Infection-Causing Bacteria and their Antibiotic Susceptibility Using Volatile Metabolite Profiles

Smart, Amy; de Lacy Costello, Ben; White, Paul; Avison, Matthew; Batty, Claire; Turner, Claire; Persaud, Raj and Ratcliffe, Norman (2019). Sniffing Out Resistance - Rapid Identification of Urinary Tract Infection-Causing Bacteria and their Antibiotic Susceptibility Using Volatile Metabolite Profiles. Journal of Pharmaceutical and Biomedical Analysis, 167 pp. 59–65.

DOI (Digital Object Identifier) Link: https://doi.org/10.1016/j.jpba.2019.01.044
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Abstract

Antibiotic resistance is set to be an unprecedented threat to modern medicine. ‘Sniffing’ bacteria potentially offers a rapid way to determine susceptibility. A successful proof-of-principle study is described, using thermal desorption-gas chromatography-mass spectrometry (TD-GC-MS) to ‘smell’ cephalexin and ciprofloxacin resistant and sensitive Urinary Tract Infection (UTI)-causing bacteria. 578 peaks at unique retention times were detected from 86 chromatograms of 18 bacterial isolates (E. coli, K. pneumoniae and P. aeruginosa). The isolates were grown with and without the presence of antibiotic. Chi-square analysis found 9 compounds that differed significantly between cephalexin sensitive and resistant isolates, and 22 compounds that differed significantly between ciprofloxacin sensitive and resistant isolates, at p ≤0.05. When antibiotic was added to the media, more differences were found in the cephalexin group, attributed to lysis, but not in the ciprofloxacin group. Further work with large sample sizes will potentially enable the development of diagnostic algorithms using presence/absence of particular compounds of interest.

Item Type: Journal Item
Copyright Holders: 2019 Elsevier B.V.
ISSN: 0731-7085
Keywords: volatile; metabolite; profiles; smell; thermal desorption; gas chromatography; mass spectrometry; antibiotic; resistance; urinary tract infection; susceptibility; cephalexin; ciprofloxacin
Academic Unit/School: Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences
Faculty of Science, Technology, Engineering and Mathematics (STEM)
Research Group: Health and Wellbeing PRA (Priority Research Area)
Item ID: 59000
Depositing User: Claire Turner
Date Deposited: 05 Feb 2019 09:04
Last Modified: 10 May 2019 15:19
URI: http://oro.open.ac.uk/id/eprint/59000
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