Copy the page URI to the clipboard
Savtchenko, Leonid P.; Bard, Lucie; Jensen, Thomas P.; Reynolds, James P.; Kraev, Igor; Medvedev, Nikolay; Stewart, Michael G.; Henneberger, Christian and Rusakov, Dmitri A.
(2018).
DOI: https://doi.org/10.1038/s41467-018-05896-w
Abstract
Electrically non-excitable astroglia take up neurotransmitters, buffer extracellular K+ and generate Ca2+ signals that release molecular regulators of neural circuitry. The underlying machinery remains enigmatic, mainly because the sponge-like astrocyte morphology has been difficult to access experimentally or explore theoretically. Here, we systematically incorporate multi-scale, tri-dimensional astroglial architecture into a realistic multi-compartmental cell model, which we constrain by empirical tests and integrate into the NEURON computational biophysical environment. This approach is implemented as a flexible astrocyte-model builder ASTRO. As a proof-of-concept, we explore an in silico astrocyte to evaluate basic cell physiology features inaccessible experimentally. Our simulations suggest that currents generated by glutamate transporters or K+ channels have negligible distant effects on membrane voltage and that individual astrocytes can successfully handle extracellular K+ hotspots. We show how intracellular Ca2+ buffers affect Ca2+ waves and why the classical Ca2+ sparks-and-puffs mechanism is theoretically compatible with common readouts of astroglial Ca2+ imaging.
Viewing alternatives
Download history
Metrics
Public Attention
Altmetrics from AltmetricNumber of Citations
Citations from DimensionsItem Actions
Export
About
- Item ORO ID
- 56588
- Item Type
- Journal Item
- ISSN
- 2041-1723
- Project Funding Details
-
Funded Project Name Project ID Funding Body Principal Fellowship 101896 Wellcome Trust Principal Fellowship 212251/ Z/18/Z Wellcome Trust Advanced Grant 323113-NETSIGNAL European Research Council ITN Extrabrain 606950 EXTRABRAIN European Union FP7 DFG Not Set German Research Foundation SFB1089 B03 Not Set German Research Foundation SPP1757 Not Set German Research Foundation HE6949/1 Not Set German Research Foundation HE6949/3 Not Set German Research Foundation ITN EUGlia Not Set European Commission Human Frontiers Science Program Not Set Not Set NRW Rückkehrerprogramm Not Set Not Set - Academic Unit or School
-
Faculty of Science, Technology, Engineering and Mathematics (STEM)
Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences - Copyright Holders
- © 2018 The Authors
- Depositing User
- Igor Kraev