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Improving cellular migration in tissue-engineered laryngeal scaffolds

Wismayer, K.; Mehrban, N.; Bowen, J. and Birchall, M. (2019). Improving cellular migration in tissue-engineered laryngeal scaffolds. Journal of Laryngology & Otology, 133(2) pp. 135–148.

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DOI (Digital Object Identifier) Link: https://doi.org/10.1017/S0022215119000082
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Abstract

Objective
To modify the non-porous surface membrane of a tissue-engineered laryngeal scaffold to allow effective cell entry.
Methods
The mechanical properties, surface topography and chemistry of polyhedral oligomeric silsesquioxane poly(carbonate-urea) urethane were characterised. A laser technique introduced surface perforations. Micro computed tomography generated porosity data. Scaffolds were seeded with cells, investigated histologically and proliferation studied. Incubation and time effects were assessed.
Results
Laser cutting perforated the polymer, connecting the substructure with the ex-scaffold environment and increasing porosity (porous, non-perforated = 87.9 per cent; porous, laser-perforated at intensities 3 = 96.4 per cent and 6 = 89.5 per cent). Cellular studies confirmed improved cell viability. Histology showed cells adherent to the scaffold surface and cells within perforations, and indicated that cells migrated into the scaffolds. After 15 days of incubation, scanning electron microscopy revealed an 11 per cent reduction in pore diameter, correlating with a decrease in Young's modulus.
Conclusion
Introducing surface perforations presents a viable method of improving polyhedral oligomeric silsesquioxane poly(carbonate-urea) urethane as a tissue-engineered scaffold.

Item Type: Journal Item
Copyright Holders: 2019 JLO Limited
ISSN: 1748-5460
Project Funding Details:
Funded Project NameProject IDFunding Body
Not SetMR/K026453/1MRC (Medical Research Council)
Not SetNot SetDivision of Surgery and Interventional Science, University College London
Keywords: Tissue Engineering; Laryngeal Neoplasms; Porosity; Cell Proliferation; Nanocomposites; Tissue Scaffolds
Academic Unit/School: Faculty of Science, Technology, Engineering and Mathematics (STEM) > Engineering and Innovation
Faculty of Science, Technology, Engineering and Mathematics (STEM)
Research Group: Smart Materials
Item ID: 56137
Depositing User: James Bowen
Date Deposited: 26 Mar 2019 11:41
Last Modified: 13 Apr 2019 01:53
URI: http://oro.open.ac.uk/id/eprint/56137
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