The Open UniversitySkip to content
 

Improving cellular migration in tissue-engineered laryngeal scaffolds

Wismayer, Kurt; Mehrban, Nazia; Bowen, James and Birchall, Martin A (2019). Improving cellular migration in tissue-engineered laryngeal scaffolds. Journal of Laryngology & Otology, 133(2) pp. 135–148.

Full text available as:
[img]
Preview
PDF (Accepted Manuscript) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
Download (1MB) | Preview
DOI (Digital Object Identifier) Link: https://doi.org/10.1017/S0022215119000082
Google Scholar: Look up in Google Scholar

Abstract

Objective
To modify the non-porous surface membrane of a tissue-engineered laryngeal scaffold to allow effective cell entry.
Methods
The mechanical properties, surface topography and chemistry of polyhedral oligomeric silsesquioxane poly(carbonate-urea) urethane were characterised. A laser technique introduced surface perforations. Micro computed tomography generated porosity data. Scaffolds were seeded with cells, investigated histologically and proliferation studied. Incubation and time effects were assessed.
Results
Laser cutting perforated the polymer, connecting the substructure with the ex-scaffold environment and increasing porosity (porous, non-perforated = 87.9 per cent; porous, laser-perforated at intensities 3 = 96.4 per cent and 6 = 89.5 per cent). Cellular studies confirmed improved cell viability. Histology showed cells adherent to the scaffold surface and cells within perforations, and indicated that cells migrated into the scaffolds. After 15 days of incubation, scanning electron microscopy revealed an 11 per cent reduction in pore diameter, correlating with a decrease in Young's modulus.
Conclusion
Introducing surface perforations presents a viable method of improving polyhedral oligomeric silsesquioxane poly(carbonate-urea) urethane as a tissue-engineered scaffold.

Item Type: Journal Item
ISSN: 1748-5460
Keywords: Tissue Engineering; Laryngeal Neoplasms; Porosity; Cell Proliferation; Nanocomposites; Tissue Scaffolds
Academic Unit/School: Faculty of Science, Technology, Engineering and Mathematics (STEM) > Engineering and Innovation
Faculty of Science, Technology, Engineering and Mathematics (STEM)
Research Group: Smart Materials
Item ID: 56137
Depositing User: James Bowen
Date Deposited: 26 Mar 2019 11:41
Last Modified: 10 Nov 2019 19:16
URI: http://oro.open.ac.uk/id/eprint/56137
Share this page:

Metrics

Altmetrics from Altmetric

Citations from Dimensions

Download history for this item

These details should be considered as only a guide to the number of downloads performed manually. Algorithmic methods have been applied in an attempt to remove automated downloads from the displayed statistics but no guarantee can be made as to the accuracy of the figures.

Actions (login may be required)

Policies | Disclaimer

© The Open University   contact the OU