Bootman, Martin D.; Allman, Sarah; Rietdorf, Katja and Bultynck, Geert
(2018).
|
PDF (Accepted Manuscript)
- Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
Download (730kB) | Preview |
|
|
PDF (Version of Record)
- Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
Download (808kB) | Preview |
| DOI (Digital Object Identifier) Link: | https://doi.org/10.1016/j.ceca.2018.04.005 |
|---|---|
| Google Scholar: | Look up in Google Scholar |
Abstract
The study of cellular Ca2+ signalling is indebted to Roger Tsien for the invention of fluorescent indicators that can be readily loaded into living cells and provide the means to measure cellular Ca2+ changes over long periods of time with sub-second resolution and microscopic precision. However, a recent study [1] reminds us that as useful as these tools are they need to be employed with caution as there can be off-target effects. This article summarises these recent findings within the wider context of confounding issues that can be encountered when using chemical and genetically-encoded Ca2+ indicators, and briefly discusses some approaches that may mitigate against misleading outcomes.
| Item Type: | Journal Item |
|---|---|
| Copyright Holders: | 2018 Elsevier Ltd. |
| ISSN: | 0143-4160 |
| Academic Unit/School: | Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences Faculty of Science, Technology, Engineering and Mathematics (STEM) |
| Item ID: | 54537 |
| Depositing User: | Martin Bootman |
| Date Deposited: | 19 Apr 2018 10:39 |
| Last Modified: | 07 Apr 2020 17:29 |
| URI: | http://oro.open.ac.uk/id/eprint/54537 |
| Share this page: |     |
Metrics
Altmetrics from Altmetric | Citations from Dimensions |
Download history for this item
These details should be considered as only a guide to the number of downloads performed manually. Algorithmic methods have been applied in an attempt to remove automated downloads from the displayed statistics but no guarantee can be made as to the accuracy of the figures.
CORE (COnnecting REpositories)
CORE (COnnecting REpositories)