The Open UniversitySkip to content
 

Deleterious effects of calcium indicators within cells; an inconvenient truth

Bootman, Martin D.; Allman, Sarah; Rietdorf, Katja and Bultynck, Geert (2018). Deleterious effects of calcium indicators within cells; an inconvenient truth. Cell Calcium, 73 pp. 82–87.

Full text available as:
[img]
Preview
PDF (Accepted Manuscript) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
Download (730kB) | Preview
[img]
Preview
PDF (Version of Record) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
Download (808kB) | Preview
DOI (Digital Object Identifier) Link: https://doi.org/10.1016/j.ceca.2018.04.005
Google Scholar: Look up in Google Scholar

Abstract

The study of cellular Ca⁠2+ signalling is indebted to Roger Tsien for the invention of fluorescent indicators that can be readily loaded into living cells and provide the means to measure cellular Ca⁠⁠2+ changes over long periods of time with sub-second resolution and microscopic precision. However, a recent study [1] reminds us that as useful as these tools are they need to be employed with caution as there can be off-target effects. This article summarises these recent findings within the wider context of confounding issues that can be encountered when using chemical and genetically-encoded Ca⁠⁠2+ indicators, and briefly discusses some approaches that may mitigate against misleading outcomes.

Item Type: Journal Item
Copyright Holders: 2018 Elsevier Ltd.
ISSN: 0143-4160
Academic Unit/School: Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences
Faculty of Science, Technology, Engineering and Mathematics (STEM)
Item ID: 54537
Depositing User: Martin Bootman
Date Deposited: 19 Apr 2018 10:39
Last Modified: 23 May 2019 09:34
URI: http://oro.open.ac.uk/id/eprint/54537
Share this page:

Metrics

Altmetrics from Altmetric

Citations from Dimensions

Download history for this item

These details should be considered as only a guide to the number of downloads performed manually. Algorithmic methods have been applied in an attempt to remove automated downloads from the displayed statistics but no guarantee can be made as to the accuracy of the figures.

Actions (login may be required)

Policies | Disclaimer

© The Open University   contact the OU