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Deleterious effects of calcium indicators within cells; an inconvenient truth

Bootman, Martin D.; Allman, Sarah; Rietdorf, Katja and Bultynck, Geert (2018). Deleterious effects of calcium indicators within cells; an inconvenient truth. Cell Calcium, 73 pp. 82–87.

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DOI (Digital Object Identifier) Link: https://doi.org/10.1016/j.ceca.2018.04.005
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Abstract

The study of cellular Ca⁠2+ signalling is indebted to Roger Tsien for the invention of fluorescent indicators that can be readily loaded into living cells and provide the means to measure cellular Ca⁠⁠2+ changes over long periods of time with sub-second resolution and microscopic precision. However, a recent study [1] reminds us that as useful as these tools are they need to be employed with caution as there can be off-target effects. This article summarises these recent findings within the wider context of confounding issues that can be encountered when using chemical and genetically-encoded Ca⁠⁠2+ indicators, and briefly discusses some approaches that may mitigate against misleading outcomes.

Item Type: Journal Item
Copyright Holders: 2018 Elsevier Ltd.
ISSN: 0143-4160
Academic Unit/School: Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences
Faculty of Science, Technology, Engineering and Mathematics (STEM)
Item ID: 54537
Depositing User: Martin Bootman
Date Deposited: 19 Apr 2018 10:39
Last Modified: 21 Sep 2018 20:28
URI: http://oro.open.ac.uk/id/eprint/54537
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