Bootman, Martin D.; Allman, Sarah; Rietdorf, Katja and Bultynck, Geert
(2018).
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(Accepted Manuscript)
Due to publisher licensing restrictions, this file is not available for public download until 15 April 2019 Click here to request a copy from the OU Author. |
| DOI (Digital Object Identifier) Link: | https://doi.org/10.1016/j.ceca.2018.04.005 |
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| Google Scholar: | Look up in Google Scholar |
Abstract
The study of cellular Ca2+ signalling is indebted to Roger Tsien for the invention of fluorescent indicators that can be readily loaded into living cells and provide the means to measure cellular Ca2+ changes over long periods of time with sub-second resolution and microscopic precision. However, a recent study [1] reminds us that as useful as these tools are they need to be employed with caution as there can be off-target effects. This article summarises these recent findings within the wider context of confounding issues that can be encountered when using chemical and genetically-encoded Ca2+ indicators, and briefly discusses some approaches that may mitigate against misleading outcomes.
| Item Type: | Journal Item |
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| Copyright Holders: | 2018 Elsevier Ltd. |
| ISSN: | 0143-4160 |
| Academic Unit/School: | Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences Faculty of Science, Technology, Engineering and Mathematics (STEM) |
| Item ID: | 54537 |
| Depositing User: | Martin Bootman |
| Date Deposited: | 19 Apr 2018 10:39 |
| Last Modified: | 16 Feb 2019 19:51 |
| URI: | http://oro.open.ac.uk/id/eprint/54537 |
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