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Long Term Potentiation (LTP) and Long Term Depression (LTD) Cause Differential Spatial Redistribution of the Synaptic Vesicle Protein Synaptophysin in the Middle Molecular Layer of the Dentate Gyrus in Rat Hippocampus

Kraev, Igor V.; Chaudhury, Sraboni; Davies, Heather A.; Dallérac, Glenn; Doyère, Valérie and Stewart, Michael G. (2016). Long Term Potentiation (LTP) and Long Term Depression (LTD) Cause Differential Spatial Redistribution of the Synaptic Vesicle Protein Synaptophysin in the Middle Molecular Layer of the Dentate Gyrus in Rat Hippocampus. Opera Medica et Physiologica, 2(3-4) pp. 205–210.

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URL: http://operamedphys.org/OMP_2016_03_0039
DOI (Digital Object Identifier) Link: https://doi.org/10.20388/omp2016.003.0039
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Abstract

The presynaptic modifications that accompany long-term changes in synaptic plasticity are still not fully understood. Synaptophysin is a major synaptic vesicle protein involved in neurotransmitter release. We have used quantitative electron microscopy to study synaptophysin (Syn) immunolabelling in the hippocampus of adult rats 24h after induction in vivo of long term potentiation (LTP), and long term depression (LTD). Electrodes were implanted chronically in hippocampus with stimulation at either the medial (MPP) or lateral perforant path (LPP). 24h following induction of LTP or LTD rats were rapidly perfusion fixed and hippocampal tissue processed to electron microscopy via freeze substitution method. Anti-synaptophysin post-embedding immunolabelling was performed and tissue was imaged in the middle molecular layer (MML) of the dentate gyrus. There was a significant decrease in number of Syn labelled vesicles per unit area of bouton after LTP, but not LTD. An analysis of the spatial distribution of Syn labelled synaptic vesicles showed an increase in nearest neighbour distances, more so in the LTP than the LTD group, which is consistent with the overall decrease of Syn after LTP. These data are in agreement with the suggestion that Syn is involved in clathrin-dependent and “kiss and run” endocytosis which occurs perisynaptically. Thus, an increase in release of neurotransmitter and in consequence endocytosis would be consistent with an increased active zone distance for vesicles containing Syn.

Item Type: Journal Item
ISSN: 2500-2287
Keywords: Synaptophysin; LTP; LTD; dentate gyrus; hippocampus; electron microscopy
Academic Unit/School: Faculty of Science, Technology, Engineering and Mathematics (STEM)
Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences
Research Group: Health and Wellbeing PRA (Priority Research Area)
Item ID: 52752
Depositing User: Igor Kraev
Date Deposited: 02 Jan 2018 11:14
Last Modified: 01 May 2019 15:45
URI: http://oro.open.ac.uk/id/eprint/52752
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