The Open UniversitySkip to content
 

Epidermis and Re-epithelialization in Schmidtea mediterranea

Gumbrys, Aurimas (2017). Epidermis and Re-epithelialization in Schmidtea mediterranea. PhD thesis The Open University.

Full text available as:
[img]
Preview
PDF (Version of Record) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
Download (16MB) | Preview
Google Scholar: Look up in Google Scholar

Abstract

Epidermal layer is crucial for organism’s survival as its ability to close the wound is essential for tissue recovery. Planarian epidermis enables animal recovery and survival after virtually any body part amputation. Nevertheless, neither the epidermis nor the mechanisms endowing such a remarkable wound healing capacity is described in detail in planarians. Our work introduces live imaging methodology, which allows following
epidermal cells and their response to tissue damage or tissue loss for extended time (hours) and in high resolution. Using our methods, we followed planarian cells live for the first time and in conjunction with electron microscopy analysis we described epidermal cell behaviors during tissue maintenance, response to tissue damage and tissue loss. Our data provides comprehensive description of cellular wound response, wound closure as well as preexisting tissue contribution to tissue restoration. In addition, we performed epidermal expression profile analysis to identify the candidate list of epidermally expressed genes to depict the machinery endowing these epidermal cell behaviors. In the pilot functional (RNAi) screen an array of transcription factors with a tissue maintenance phenotypes were identified. Our work established tools for subsequent functional studies of other epidermal
expressed genes and paved the way to dissect the mechanisms of the epidermis’ maintenance and efficient wound healing in planarians.

Item Type: Thesis (PhD)
Copyright Holders: 2016 The Author
Keywords: planaria; epidermis; regeneration (biology); wound healing
Academic Unit/School: Faculty of Science, Technology, Engineering and Mathematics (STEM)
Associated Research Centre: Stowers Institute for Medical Research
Item ID: 50972
Depositing User: Aurimas Gumbrys
Date Deposited: 16 Oct 2017 10:39
Last Modified: 20 Oct 2018 19:37
URI: http://oro.open.ac.uk/id/eprint/50972
Share this page:

Download history for this item

These details should be considered as only a guide to the number of downloads performed manually. Algorithmic methods have been applied in an attempt to remove automated downloads from the displayed statistics but no guarantee can be made as to the accuracy of the figures.

Actions (login may be required)

Policies | Disclaimer

© The Open University   contact the OU