Norris, Vic; Krylov, Sergey N.; Agarwal, Pratul K. and White, Glenn J.
(2017).
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| DOI (Digital Object Identifier) Link: | https://doi.org/10.1159/000464443 |
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Abstract
The construction of switchable, radiation-controlled, aptameric enzymes – “swenzymes” – is, in principle, feasible. We propose a strategy to make such catalysts from 2 (or more) aptamers each selected to bind specifically to one of the substrates in, for example, a 2-substrate reaction. Construction of a combinatorial library of candidate swenzymes entails selecting a set of a million aptamers that bind one substrate and a second set of a million aptamers that bind the second substrate; the aptamers in these sets are then linked pairwise by a linker, thus bringing together the substrates. In the presence of the substrates, some linked aptamer pairs catalyze the reaction when exposed to external energy in the form of a specific frequency of low intensity, nonionizing electromagnetic or acoustic radiation. Such swenzymes are detected via a separate product-capturing aptamer that changes conformation on capturing the product; this altered conformation allows it (1) to bind to every potential swenzyme in its vicinity (thereby giving a higher probability of capture to the swenzymes that generate the product) and (2) to bind to a sequence on a magnetic bead (thereby permitting purification of the swenzyme plus product-capturing aptamer by precipitation). Attempts to implement the swenzyme strategy may help elucidate fundamental problems in enzyme catalysis.
| Item Type: | Journal Item |
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| Copyright Holders: | 2017 S. Karger AG |
| ISSN: | 1660-2412 |
| Keywords: | catalyst; catalytic antibody; ribozyme; enzyme; aptamer; synthetic biology; radiation; sound; light |
| Academic Unit/School: | Faculty of Science, Technology, Engineering and Mathematics (STEM) > Physical Sciences Faculty of Science, Technology, Engineering and Mathematics (STEM) |
| Item ID: | 48669 |
| Depositing User: | G. J. White |
| Date Deposited: | 27 Mar 2017 14:15 |
| Last Modified: | 16 Jun 2019 08:11 |
| URI: | http://oro.open.ac.uk/id/eprint/48669 |
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