Norris, Vic; Krylov, Sergey N.; Agarwal, Pratul K. and White, Glenn J.
(2017).
|
PDF (Version of Record)
- Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
Download (875kB) | Preview |
| DOI (Digital Object Identifier) Link: | https://doi.org/10.1159/000464443 |
|---|---|
| Google Scholar: | Look up in Google Scholar |
Abstract
The construction of switchable, radiation-controlled, aptameric enzymes – “swenzymes” – is, in principle, feasible. We propose a strategy to make such catalysts from 2 (or more) aptamers each selected to bind specifically to one of the substrates in, for example, a 2-substrate reaction. Construction of a combinatorial library of candidate swenzymes entails selecting a set of a million aptamers that bind one substrate and a second set of a million aptamers that bind the second substrate; the aptamers in these sets are then linked pairwise by a linker, thus bringing together the substrates. In the presence of the substrates, some linked aptamer pairs catalyze the reaction when exposed to external energy in the form of a specific frequency of low intensity, nonionizing electromagnetic or acoustic radiation. Such swenzymes are detected via a separate product-capturing aptamer that changes conformation on capturing the product; this altered conformation allows it (1) to bind to every potential swenzyme in its vicinity (thereby giving a higher probability of capture to the swenzymes that generate the product) and (2) to bind to a sequence on a magnetic bead (thereby permitting purification of the swenzyme plus product-capturing aptamer by precipitation). Attempts to implement the swenzyme strategy may help elucidate fundamental problems in enzyme catalysis.
| Item Type: | Journal Item |
|---|---|
| Copyright Holders: | 2017 S. Karger AG |
| ISSN: | 1660-2412 |
| Keywords: | catalyst; catalytic antibody; ribozyme; enzyme; aptamer; synthetic biology; radiation; sound; light |
| Academic Unit/School: | Faculty of Science, Technology, Engineering and Mathematics (STEM) > Physical Sciences Faculty of Science, Technology, Engineering and Mathematics (STEM) |
| Item ID: | 48669 |
| Depositing User: | G. J. White |
| Date Deposited: | 27 Mar 2017 14:15 |
| Last Modified: | 17 Oct 2019 05:55 |
| URI: | http://oro.open.ac.uk/id/eprint/48669 |
| Share this page: |     |
Metrics
Altmetrics from Altmetric | Citations from Dimensions |
Download history for this item
These details should be considered as only a guide to the number of downloads performed manually. Algorithmic methods have been applied in an attempt to remove automated downloads from the displayed statistics but no guarantee can be made as to the accuracy of the figures.
CORE (COnnecting REpositories)
CORE (COnnecting REpositories)