East, Emma; Johns, Noémie; Georgiou, Melanie; Golding, Jon P.; Loughlin, A. Jane; Kingham, Paul J. and Phillips, James B.
(2013).
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| DOI (Digital Object Identifier) Link: | https://doi.org/10.2217/RME.13.61 |
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| Google Scholar: | Look up in Google Scholar |
Abstract
Aim: This study aimed to develop a 3D culture model to test the extent to which transplanted stem cells modulate astrocyte reactivity, where exacerbated glial cell activation could be detrimental to CNS repair success.
Materials & methods: The reactivity of rat astrocytes to bone marrow mesenchymal stem cells, neural crest stem cells (NCSCs) and differentiated adipose-derived stem cells was assessed after 5 days. Schwann cells were used as a positive control.
Results: NCSCs and differentiated Schwann cell-like adipose-derived stem cells did not increase astrocyte reactivity. Highly reactive responses to bone marrow mesenchymal stem cells and Schwann cells were equivalent.
Conclusion: This approach can screen therapeutic cells prior to in vivo testing, allowing cells likely to trigger a substantial astrocyte response to be identified at an early stage. NCSCs and differentiated Schwann cell-like adipose-derived stem cells may be useful in treating CNS damage without increasing astrogliosis.
| Item Type: | Journal Item |
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| Copyright Holders: | 2013 The Authors |
| ISSN: | 1746-076X |
| Keywords: | 3D cell culture; astrocytes; CNS; reactive gliosis; spinal cord; stem cell therapy |
| Academic Unit/School: | Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences Faculty of Science, Technology, Engineering and Mathematics (STEM) |
| Related URLs: |
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| Item ID: | 46743 |
| Depositing User: | Jon Golding |
| Date Deposited: | 04 Jul 2016 09:43 |
| Last Modified: | 07 Dec 2018 12:20 |
| URI: | http://oro.open.ac.uk/id/eprint/46743 |
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