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East, Emma; Johns, Noémie; Georgiou, Melanie; Golding, Jon P.; Loughlin, A. Jane; Kingham, Paul J. and Phillips, James B.
(2013).
DOI: https://doi.org/10.2217/RME.13.61
Abstract
Aim: This study aimed to develop a 3D culture model to test the extent to which transplanted stem cells modulate astrocyte reactivity, where exacerbated glial cell activation could be detrimental to CNS repair success.
Materials & methods: The reactivity of rat astrocytes to bone marrow mesenchymal stem cells, neural crest stem cells (NCSCs) and differentiated adipose-derived stem cells was assessed after 5 days. Schwann cells were used as a positive control.
Results: NCSCs and differentiated Schwann cell-like adipose-derived stem cells did not increase astrocyte reactivity. Highly reactive responses to bone marrow mesenchymal stem cells and Schwann cells were equivalent.
Conclusion: This approach can screen therapeutic cells prior to in vivo testing, allowing cells likely to trigger a substantial astrocyte response to be identified at an early stage. NCSCs and differentiated Schwann cell-like adipose-derived stem cells may be useful in treating CNS damage without increasing astrogliosis.
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About
- Item ORO ID
- 46743
- Item Type
- Journal Item
- ISSN
- 1746-076X
- Keywords
- 3D cell culture; astrocytes; CNS; reactive gliosis; spinal cord; stem cell therapy
- Academic Unit or School
-
Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences
Faculty of Science, Technology, Engineering and Mathematics (STEM) - Copyright Holders
- © 2013 The Authors
- Related URLs
-
- http://www.jamesphillips.org(Author Website)
- Depositing User
- Jon Golding