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Wan Dagang, W.R.Z.; Bowen, J.; O'Keeffe, J.; Robbins, P.T. and Zhang, Z.
(2016).
DOI: https://doi.org/10.1007/s10529-016-2047-x
Abstract
Objectives: The adhesion of colloidal probes of stainless steel, glass and cellulose to Pseudomonas fluorescens biofilms was examined using atomic force microscopy (AFM) to allow comparisons between surfaces to which biofilms might adhere. Results: Biofilm was grown on a stainless steel substrate and covered most of the surface after 96 h. AFM approach and retraction curves were obtained when the biofilm was immersed in a tryptone/soy medium. On approach, all the colloidal probes experienced a long non-contact phase more than 100 nm in length, possibly due to the steric repulsion by extracellular polymers from the biofilm and hydrophobic effects. Retraction data showed that the adhesion varied from position to position on the biofilm. The mean value of adhesion of glass to the biofilm (48 ± 7 nN) was the greatest, followed by stainless steel (30 ± 7 nN) and cellulose (7.8 ± 0.4 nN). Conclusion: The method allows understanding of adhesion between the three materials and biofilm, and development of a better strategy to remove the biofilm from these surfaces relevant to different industrial applications.
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About
- Item ORO ID
- 45977
- Item Type
- Journal Item
- ISSN
- 1573-6776
- Project Funding Details
-
Funded Project Name Project ID Funding Body Fundamental Research Grant Scheme R.J130000.7845.4F428 Malaysia of Education Malaysia (MOE), Universiti Teknologi Malaysia Not Set Not Set Unilever R&D - Keywords
- Adhesion; Atomic force microscopy; Biofilm; Colloidal probe; Pseudomonas fluorescens; Repulsion
- Academic Unit or School
-
Faculty of Science, Technology, Engineering and Mathematics (STEM) > Engineering and Innovation
Faculty of Science, Technology, Engineering and Mathematics (STEM) - Copyright Holders
- © 2016 Springer
- Depositing User
- James Bowen