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Adhesion of Pseudomonas fluorescens biofilms to glass, stainless steel and cellulose

Wan Dagang, W.R.Z.; Bowen, J.; O'Keeffe, J.; Robbins, P.T. and Zhang, Z. (2016). Adhesion of Pseudomonas fluorescens biofilms to glass, stainless steel and cellulose. Biotechnology Letters, 38(5) pp. 787–792.

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DOI (Digital Object Identifier) Link: https://doi.org/10.1007/s10529-016-2047-x
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Abstract

Objectives: The adhesion of colloidal probes of stainless steel, glass and cellulose to Pseudomonas fluorescens biofilms was examined using atomic force microscopy (AFM) to allow comparisons between surfaces to which biofilms might adhere. Results: Biofilm was grown on a stainless steel substrate and covered most of the surface after 96 h. AFM approach and retraction curves were obtained when the biofilm was immersed in a tryptone/soy medium. On approach, all the colloidal probes experienced a long non-contact phase more than 100 nm in length, possibly due to the steric repulsion by extracellular polymers from the biofilm and hydrophobic effects. Retraction data showed that the adhesion varied from position to position on the biofilm. The mean value of adhesion of glass to the biofilm (48 ± 7 nN) was the greatest, followed by stainless steel (30 ± 7 nN) and cellulose (7.8 ± 0.4 nN). Conclusion: The method allows understanding of adhesion between the three materials and biofilm, and development of a better strategy to remove the biofilm from these surfaces relevant to different industrial applications.

Item Type: Journal Item
Copyright Holders: 2016 Springer
ISSN: 1573-6776
Project Funding Details:
Funded Project NameProject IDFunding Body
Fundamental Research Grant SchemeR.J130000.7845.4F428Malaysia of Education Malaysia (MOE), Universiti Teknologi Malaysia
Not SetNot SetUnilever R&D
Keywords: Adhesion; Atomic force microscopy; Biofilm; Colloidal probe; Pseudomonas fluorescens; Repulsion
Academic Unit/School: Faculty of Science, Technology, Engineering and Mathematics (STEM) > Engineering and Innovation
Faculty of Science, Technology, Engineering and Mathematics (STEM)
Item ID: 45977
Depositing User: James Bowen
Date Deposited: 08 Apr 2016 11:35
Last Modified: 18 Feb 2017 02:56
URI: http://oro.open.ac.uk/id/eprint/45977
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