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A mouse model of the 15q13.3 microdeletion syndrome shows prefrontal neurophysiological dysfunctions and attentional impairment.

Nilsson, Simon R. O.; Celada, Pau; Fejgin, Kim; Thelin, Jonas; Nielsen, Jacob; Santana, Noemí; Heath, Christopher J.; Larsen, Peter H.; Nielsen, Vibeke; Kent, Brianne A.; Saksida, Lisa M.; Stensbøl, Tine B.; Robbins, Trevor W.; Bastlund, Jesper F.; Bussey, Timothy J.; Artigas, Francesc and Didriksen, Michael (2016). A mouse model of the 15q13.3 microdeletion syndrome shows prefrontal neurophysiological dysfunctions and attentional impairment. Psychopharmacology

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DOI (Digital Object Identifier) Link: https://doi.org/10.1007/s00213-016-4265-2
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Abstract

Rationale
A microdeletion at locus 15q13.3 is associated with high incidence rates of psychopathology, including schizophrenia. A mouse model of the 15q13.3 microdeletion syndrome has been generated (Df[h15q13]/+) with translational utility for modelling schizophrenia-like pathology. Among other deficits, schizophrenia is characterised by dysfunctions in prefrontal cortical (PFC) inhibitory circuitry and attention.

Objectives
The objective of this study is to assess PFC-dependent functioning in the Df(h15q13)/+ mouse using electrophysiological, pharmacological, and behavioural assays.

Method
Experiments 1–2 investigated baseline firing and auditory-evoked responses of PFC interneurons and pyramidal neurons. Experiment 3 measured pyramidal firing in response to intra-PFC GABAA receptor antagonism. Experiments 4–6 assessed PFC-dependent attentional functioning through the touchscreen 5-choice serial reaction time task (5-CSRTT). Experiments 7–12 assessed reversal learning, paired-associate learning, extinction learning, progressive ratio, trial-unique non-match to sample, and object recognition.

Results
In experiments 1–3, the Df(h15q13)/+ mouse showed reduced baseline firing rate of fast-spiking interneurons and in the ability of the GABAA receptor antagonist gabazine to increase the firing rate of pyramidal neurons. In assays of auditory-evoked responses, PFC interneurons in the Df(h15q13)/+ mouse had reduced detection amplitudes and increased detection latencies, while pyramidal neurons showed increased detection latencies. In experiments 4–6, the Df(h15q13)/+ mouse showed a stimulus duration-dependent decrease in percent accuracy in the 5-CSRTT. The impairment was insensitive to treatment with the partial α7nAChR agonist EVP-6124. The Df(h15q13)/+ mouse showed no cognitive impairments in experiments 7–12.

Conclusion
The Df(h15q13)/+ mouse has multiple dysfunctions converging on disrupted PFC processing as measured by several independent assays of inhibitory transmission and attentional function.

Item Type: Journal Item
Copyright Holders: 2016 The Authors
ISSN: 1432-2072
Keywords: copy number variation; 15q13.3; animal model; Chrna7; prefrontal cortex; neurophysiology; cognition
Academic Unit/School: Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences
Faculty of Science, Technology, Engineering and Mathematics (STEM)
Item ID: 45754
Depositing User: Christopher Heath
Date Deposited: 21 Mar 2016 10:02
Last Modified: 23 May 2019 20:08
URI: http://oro.open.ac.uk/id/eprint/45754
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