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Motivational assessment of mice using the touchscreen operant testing system: effects of dopaminergic drugs

Heath, Christopher J.; Bussey, Timothy J. and Saksida, Lisa M. (2015). Motivational assessment of mice using the touchscreen operant testing system: effects of dopaminergic drugs. Psychopharmacology, 232(21) pp. 4043–4057.

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DOI (Digital Object Identifier) Link: https://doi.org/10.1007/s00213-015-4009-8
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Abstract

Rationale
Touchscreens are widely used to examine rodent cognition. Current paradigms require animals to view stimuli and nose poke at an appropriate touchscreen location. After responding, there is little screen interaction and, as infra-red touchscreens eliminate the need for physical contact, minimal somatosensory feedback. It is therefore unclear if touchscreens can support the vigorous, repetitive responding required in paradigms like progressive ratio (PR) for assessing motivation and effort-related choice (ERC) for assessing decision-making.

Objectives
This study aims to adapt and validate PR and ERC for the rodent touchscreen.

Methods
Male C57Bl/6 mice were trained until responding on PR stabilised. Amphetamine, sulpiride and raclopride were administered via the intraperitoneal route to modify performance. Mice were transferred to ERC and paradigm parameters adjusted to demonstrate behavioural modification. ERC reward preference was assessed by home cage choice analysis.

Results
PR performance stabilised within seven sessions. Amphetamine (1 mg/kg) increased and raclopride (0.3 mg/kg) decreased performance by 63 and 28 %, respectively, with a 20-min injection-test interval. Sulpiride (50 mg/kg) decreased performance by 19 % following a 40-min injection-test interval. Increasing ERC operant requirements shifted responding from the operant response-dependent preferred reward towards the freely available alternative.

Conclusions
Vigorous, repetitive responding is sustainable in touchscreen PR and ERC and task validation mirrors non-touchscreen versions. Thus, motivation and reward-related decision-making can be measured directly with touchscreens and can be evaluated prior to cognitive testing in the same apparatus to avoid confounding by motivational factors.

Item Type: Journal Item
Copyright Holders: 2015 The Authors
ISSN: 1432-2072
Keywords: touchscreen; rodent; motivation; progressive ratio; effort-related choice; dopamine
Academic Unit/School: Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences
Faculty of Science, Technology, Engineering and Mathematics (STEM)
Item ID: 44142
Depositing User: Christopher Heath
Date Deposited: 30 Sep 2015 09:07
Last Modified: 08 Dec 2018 12:35
URI: http://oro.open.ac.uk/id/eprint/44142
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