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Insulin-like growth factor-II/Mannose-6-phosphate receptor in the spinal cord and dorsal root ganglia of the adult rat

Hawkes, Cheryl and Kar, S. (2002). Insulin-like growth factor-II/Mannose-6-phosphate receptor in the spinal cord and dorsal root ganglia of the adult rat. European Journal of Neuroscience, 15(1) pp. 33–39.

DOI (Digital Object Identifier) Link: https://doi.org/10.1046/j.0953-816x.2001.01864.x
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Abstract

The insulin-like growth factor-II/mannose-6-phosphate (IGF-II/M6P) receptor is a multifunctional transmembrane glycoprotein, which interacts with a number of molecules, including IGF-II and M6P-containing lysosomal enzymes. The receptor is widely distributed throughout the brain and is known to be involved in lysosomal enzyme trafficking, cell growth, internalization and degradation of IGF-II. In the present study, using autoradiographic, Western blotting and immunocytochemical methods, we provide the first report that IGF-II/M6P receptors are discretely distributed at all major segmental levels of the spinal cord and dorsal root ganglia of the adult rat. In the spinal cord, a high density of [125I]IGF-II binding sites was evident in the ventral horn (lamina IX) and in areas around the central canal (lamina X), whereas intermediate grey matter and dorsal horn were associated with moderate receptor levels. The dorsal root ganglia exhibited rather high density of [125I]IGF-II binding sites. Interestingly, meninges present around the spinal cord displayed highest density of [125I]IGF-II binding compared to any given region of the spinal grey mater or the dorsal root ganglia. Western blot results indicated the presence of the IGF-II/M6P receptor at all major levels of spinal cord and dorsal root ganglia, with little segmental variation. At the cellular level, spinal motorneurons demonstrated the most intense IGF-II/M6P receptor immunoreactivity, followed by interneurons in the intermediate region and deeper dorsal horn. Some scattered IGF-II/M6P immunoreactive fibers were found in the superficial laminae of the dorsal horn and dorsolateral funiculus. The meninges of the spinal cord also seemed to express IGF-II receptor immunoreactivity. In the dorsal root ganglia, receptor immunoreactivity was evident primarily in a subset of neurons of all diameters. These results, taken together, provide anatomical evidence of a role for the IGF-II/M6P receptor in general cellular functions such as transport of lysosomal enzymes and/or internalization followed by clearance of IGF-II in the spinal cord and dorsal root ganglia.

Item Type: Journal Item
Copyright Holders: 2002 Federation of European Neuroscience Societies
ISSN: 0953-816X
Project Funding Details:
Funded Project NameProject IDFunding Body
Not SetNot SetNatural Sciences and Engineering Research Council (NSERC) of Canada
Extra Information: cited By 10
Keywords: dorsal horn; immunocytochemistry; motorneurons; receptor autoradiography; sensory neurons; Western blot
Academic Unit/School: Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences
Faculty of Science, Technology, Engineering and Mathematics (STEM)
Item ID: 44113
Depositing User: Cheryl Hawkes
Date Deposited: 20 Aug 2015 14:16
Last Modified: 07 Dec 2018 10:34
URI: http://oro.open.ac.uk/id/eprint/44113
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