The Open UniversitySkip to content
 

Insulin-like growth factor-II/mannose-6-phosphate receptor: Widespread distribution in neurons of the central nervous system including those expressing cholinergic phenotype

Hawkes, Cheryl and Kar, Satyabrata (2003). Insulin-like growth factor-II/mannose-6-phosphate receptor: Widespread distribution in neurons of the central nervous system including those expressing cholinergic phenotype. Journal of Comparative Neurology, 458(2) pp. 113–127.

DOI (Digital Object Identifier) Link: https://doi.org/10.1002/cne.10578
Google Scholar: Look up in Google Scholar

Abstract

The insulin-like growth factor-II/mannose-6-phosphate (IGF-II/M6P) receptor is single transmembrane glycoprotein that plays a critical role in the trafficking of lysosomal enzymes and the internalization of circulating IGF-II. At present, there is little information regarding the cellular distribution of the IGF-II/M6P receptor within the adult rat brain. With the use of immunoblotting and immunocytochemical methods, we found that the IGF-II/M6P receptor is widely but selectively expressed in all major brain areas, including the olfactory bulb, striatum, cortex, hippocampus, thalamus, hypothalamus, cerebellum, brainstem, and spinal cord. Intense IGF-II/M6P receptor immunoreactivity was apparent on neuronal cell bodies within the striatum, deeper layers (layers IV and V) of the cortex, pyramidal and granule cell layers of the hippocampal formation, selected thalamic nuclei, Purkinje cells of the cerebellum, pontine nucleus and motoneurons of the brainstem as well as in the spinal cord. Moderate neuronal labeling was evident in the olfactory bulb, basal forebrain areas, hypothalamus, superior colliculus, midbrain areas, granule cells of the cerebellum and in the intermediate regions of the spinal gray matter. We also observed dense neuropil labeling in many regions, suggesting that this receptor is localized in dendrites and/or axon terminals. Double-labeling studies further indicated that a subset of IGF-II/M6P receptor colocalizes with cholinergic cell bodies and fibers in the septum, striatum, diagonal band complex, nucleus basalis, cortex, hippocampus, and motoneurons of the brainstem and spinal cord. The observed widespread distribution and colocalization of IGF-II/M6P receptor in the adult rat brain provide an anatomic basis to suggest a multifunctional role for the receptor in a wide-spectrum of central nervous system neurons, including those expressing a cholinergic phenotype.

Item Type: Journal Item
Copyright Holders: 2003 Wiley-Liss, Inc.
ISSN: 1096-9861
Project Funding Details:
Funded Project NameProject IDFunding Body
Not Set203518-01Natural Sciences and Engineering Research Council of Canada
Not SetMOP-43991Canadian Institutes of Health Research
Not SetChercheur-Boursier Senior awardFonds de la Recherche en Santé du Quebéc
Extra Information: cited By 35
Keywords: brain; insulin-like growth factors; immunocytochemistry; western blot
Academic Unit/School: Faculty of Science, Technology, Engineering and Mathematics (STEM) > Life, Health and Chemical Sciences
Faculty of Science, Technology, Engineering and Mathematics (STEM)
Item ID: 44112
Depositing User: Cheryl Hawkes
Date Deposited: 20 Aug 2015 14:23
Last Modified: 07 Dec 2018 10:34
URI: http://oro.open.ac.uk/id/eprint/44112
Share this page:

Metrics

Altmetrics from Altmetric

Citations from Dimensions

Actions (login may be required)

Policies | Disclaimer

© The Open University   contact the OU